极地研究

极地研究 ›› 2024, Vol. 36 ›› Issue (4): 568-580.DOI: 10.13679/j.jdyj.20230060

• 研究论文 • 上一篇    下一篇

南极菲尔德斯半岛地区不同生境中可培养细菌的多样性

郝墣钰1,张义和2,韩玮2,曾胤新2   

  1. 1自然资源部极地科学重点实验室, 中国极地研究中心(中国极地研究所), 上海 200136;  
    2上海交通大学海洋学院, 上海 200030; 
    3河南农业大学资源与环境学院, 河南 郑州 450046

  • 收稿日期:2023-09-15 修回日期:2023-12-11 出版日期:2024-12-31 发布日期:2025-01-15
  • 通讯作者: 郝墣钰
  • 作者简介:郝墣钰, 女, 1997年生。硕士研究生, 研究方向为海洋生物学。E-mail: 18503887677@163.com
  • 基金资助:
    基于组学技术的南极企鹅食性研究;极地微生物资源特性与培养技术

Diversity of culturable bacteria in soils, penguin ornithogenic soils and intertidal sediments of the Fildes Peninsula region, Antarctic#br#

HAO Puyu1,3, ZHANG Yihe1, HAN Wei1, ZENG Yinxin1,2   

  1. 1 Key Laboratory of Polar Science, Ministry of Natural Resources, Polar Research Institute of China, Shanghai 200136, China;
    2 School of Oceanography, Shanghai Jiao Tong University, Shanghai 200030, China;
    3 College of Resource and Environment, Henan Agricaltural University, Zhengzhou 450046, China

  • Received:2023-09-15 Revised:2023-12-11 Online:2024-12-31 Published:2025-01-15
  • Contact: Pu-Yu HAO

PDF (PC)

161

摘要：  菲尔德斯半岛地区具有丰富多样的微生物, 这些微生物不但在当地发挥着重要的生态学作用, 而且也是潜在、新颖的微生物资源。本研究采用传统分离培养手段, 对来源于南极菲尔德斯半岛地区的原始土壤、企鹅粪土及海洋潮间带3类不同生境中的细菌进行分离培养, 并对其开展了基于16S rRNA基因的分子鉴定。共分离得到分布于4个门、51个属的408株细菌, 其中8株菌为潜在新种。结果显示, 不同生境中可培养优势菌的组成存在明显差别, 如原始土壤中放线菌占据明显优势, 潮间带中拟杆菌占据绝对优势。相对于Luria-Bertani及Yeast extract-Peptone-Glycerol培养基, 采用寡营养培养基Reasoner’s 2A从陆地样品中获得的分离菌株的多样性更高。本文的研究结果不但能够增进对南极细菌多样性的认识, 而且可进一步丰富我国的极地微生物资源。

关键词: 可培养细菌, 多样性, 新种, 菲尔德斯半岛, 阿德雷岛, 南极

Abstract: The Fildes Peninsula region is rich in diverse microorganisms, which play important ecological roles in local biogenic element cycling and energy flow, and are potential and novel resources for microorganisms. In this study, bacteria from soils, penguin ornithogenic soils and intertidal sediments collected in the Antarctic Fildes Peninsula region were isolated using traditional cultivation techniques, and then classified based on 16S rRNA gene sequencing. A total of 408 bacterial strains were isolated, falling into four bacterial phyla and 51 genera; of these, eight bacterial strains were potential novel species. Differences in the composition of the dominant bacterial groups were observed among soils, penguin ornithogenic soils and intertidal sediments. Actinobacteria dominated in soils, whereas Bacteroidetes was predominant in intertidal sediments. Compared with Luria-Bertani (LB) and Yeast extract-Peptone-Glycerol (YPG) media, higher diversity was observed in cultivated bacteria isolated from terrestrial samples based on the oligotrophic Reasoner’s 2A (R2A) medium. The results of this study improve our understanding of bacterial diversity in the Antarctic and provide more materials for scientific research.

Key words: Culturable bacteria, Diversity, Novel species, Fildes Peninsula Region, Ardley Island, Antarctic