ADVANCES IN POLAR SCIENCE ›› 2013, Vol. 25 ›› Issue (3): 257-267.DOI: 10.3724/SP.J.2013.00257

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STUDIES ON RESPONSE OF ARCTIC MICROALGAE TO CHANGE OF IRRADIANCE,  APPLYING TWO KINDS OF FLUORESCENT TECHNIQUES

Wang Xiaoying1,2,He Jianfeng1,Guang Yingzhi1,Li Juanying2,Zhang Fang1   

  1. 1 SOA Key Laboratory for Polar Science,Polar Research Institute of China, Shanghai 200136, China; 2 College of Fisheries and life science, Shanghai Ocean University, Shanghai 201306,China
  • Received:2013-01-04 Revised:2013-04-25 Online:2013-09-30 Published:2013-09-30
  • Contact: Fang Zhang

Abstract: This work investigated fluorescence characteristic of two kinds of arctic algae (Heterocapsa arctica and Rhodomonas sp.) by two kinds of fluorescence techniques in vivo including Pulse-Amplitude-Modulation (PAM) and fluorescence spectrum. The main results are as follows: (1) the photosynthetic physiology of two kinds of algae were similar under the three kinds of irradiance (5000 lux 15000 lux and 25000 lux); the irradiance is stronger, the value of the Fv/Fm and photosynthetic efficiency are higher; under 25000 lux, they were in stress state, but their endurance capacity was enhanced. (2) the fluorescence intensity of Chl a of the two kinds of algae is highest under 15000lux; the fluorescence intensity of Chl a reduced on 15000lux; Carotenoids and Peridinins were the effective pigments for the survival of Heterocapsa arctica under 15000lux; phycobilin and Chl c had a positive effect on maintain the existence of Rhodomonas sp. under 5000 lux and 15000 lux.

Key words: Arctic microalgae, irradiance, pulse-amplitude-modulation(PAM), ChlorophyⅡ, fluorometer, fluorescence spectrum