Abstract:

Four SOD-producting bacteria NJ062,NJ379,NJ522 and NJ548 were selected from 421 Antarctic bacteria.They belonged to psychrophile for their optimum growth temperatures were about 10—15℃ and they could grow at 0 ℃ and they only contained one kind of SOD isoenzymes.The SOD of NJ062 was Mn-SOD,while the SODs of other three bacteria were Fe-SOD.The four SOD's activity at 0 ℃ was nearly 20%—35% of the maximum activity,and their maximal activity temperature was 40—50 ℃.In addition,the four SODs were unstable to higher temperature indicating that they were cold-active enzymes.Four Antarctic psychrophile NJ062,NJ379,NJ522 and NJ548 were identified by 16S rDNA sequence analysis and result showed that all of these four strains belonged to γ-Proteobacteria in Bacteria.The strain NJ062 was determined as genus Pseudoalteromonas sp.,the strain NJ379 belonged to genus Shewanella sp.,and the strain NJ522 and NJ548 were classified as Marinomonas sp..