Abstract:

Denaturing gradient gel electrophoresis (DGGE) profiles of PCR amplified V3 regions of 16S rDNA were used to assess bacterial diversity in the topsoil samples collected in the vicinity of the Great Wall Station, Antarctica. DGGE profile showed that bacterial phylogenetic composition varied in different soils. Sequences corresponding to 12 dominating excised bands were analyzed and fell into eight lineages of the domain Bacteria: β-, γ-, δ- proteobacteria; Cytophaga-Flexibacter-Bacteroides(CFB) group; Actinobacteria; Acidobacteria; Cyanobacteria and green nonsulfur bacteria. The majority of the sequences were most closely related to the sequences from uncultured microorganisms in environmental (especially soils and sediments).