极地研究

极地研究 ›› 2009, Vol. 21 ›› Issue (1): 15-24.

• 研究论文 • 上一篇    下一篇

白令海北部表层沉积物中细菌多样性的研究

邹扬, 曾胤新, 田蕴, 郑天凌   

  1. 厦门大学生命科学学院; 滨海湿地生态系统教育部重点实验室; 厦门大学; 近海海洋环境科学国家重点实验室; 中国极地研究中心; 国家海洋局极地科学重点实验室
  • 出版日期:2009-03-30 发布日期:2009-03-30
  • 基金资助:

    国家自然科学基金(项目编号40676002);; 国际极地年中国行动计划;; 长江学者和创新团队发展计划项目(40521003)资助

INVESTIGATION OF BACTERIAL DIVERSITY IN SURFACE SEDIMENT FROM THE NORTHERN BERING SEA

JU Yang, CENG Yin-Xin, TIAN Wen, ZHENG Tian-Ling   

  1. 1 Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystem; School of Life Sciences; Xiamen University; Xiamen 361005; China; 2 Key Laboratory for Polar Science of State Oceanic Administration; Polar Research Institute of China; Shanghai 200136; 3 State Key Laboratory of Marine Environmental Science; China
  • Online:2009-03-30 Published:2009-03-30

PDF (PC)

1750

被引次数

26

摘要：

提取白令海表层沉积物样品总DNA,构建沉积物中的细菌16SrDNA文库,通过RFLP酶切分型与序列测定,进行沉积物中的细菌多样性及系统发育分析。结果表明,该沉积物中细菌多样性丰富,获得的125条序列归属于10个细菌类群,包括占据优势地位的变形细菌(Pro-teobacteria,49.6%)、酸杆菌门(Acidobacteria,20.8%),以及拟杆菌门(Bacteroidetes,6.4%)、放线菌门(Actinobacteria,5.6%)、浮霉菌门(Planctomycetes,5.6%)、硝化螺旋菌门(Nitrospirae,4%)、疣微菌门(Verrucomicrobia,2.4%)、绿弯菌门(Chloroflexi,1.6%)、纤维杆菌门(Fibrobacteres,0.8%)、螺旋体(Spirochaetes,0.8%)。此外,还有一部分分类地位尚不明确的细菌(2.4%)。而在变形细菌中,δ、γ亚群为其中的优势类群。

关键词: 白令海, 沉积物, 细菌, 多样性, 白令海, 沉积物, 细菌, 多样性

Abstract:

Biodiversity of bacteria in the top layer of the sediment from Northern Bering Sea was studied by a combination of RFLP and 16S rDNA analysis. Phylogenetic analysis showed that bacterial community composition of the sediment had a high diversity. 124 clones from the 16S rDNA library fell into ten bacterial groups,including Proteobacteria (49.6%),Acidobacteria (20.8%),Bacteroidetes (6.4%),Actinobacteria (5.6%),Planctomycetes (5.6%),Nitrospirae (4%),Verrucomicrobia (2.4%),Chloroflexi (1.6%),Fibrobacteres (0.8...

Key words: Northern Bering Sea, sediments, bacteria, diversity