COMMUNITY COMPOSITION OF ANTARCTIC BACTERIOPLANKTON ISOLATED FROM THE PRYDZ BAY, ANTARCTICA

doi:10.13679/j.jdyj.2014.2.222

Abstract

Abstract: Twelve sea water samples of three stations from the Antarctic Prydz Bay, near Cape Darnley, were collected. Based on the oligographic media, totally 95 strains of antarctic bacterioplankton were isolated. The 16S rRNA gene sequences were determined and analyzed. The isolates fell into three lineages: Alphaproteobactia, Gammaproteobacteria and Bacteroidetes. The sequences were assigned to 35 operational taxonomic units (OTUs) based on sequence analysis. The results revealed that the Alphaproteobactia was the dominant bacterial groups and the proportions were 52.6~62.5% in different layers. Analysis using shannon diversity index indicated that station P2-14 located in continental shelf was higher than station P2-11 and P2-8 located in Slope area. There was a positive correlation between the bacteria diversity and biomass as well as productivity of phytoplankton. Shannon diversity index of the surface is the lowest, and Shannon diversity index of 50 m layer is highest. There was no difference of Shannon diversity index between 100 and 200 m layers. Bacteria vertical distribution is correlated to the nutrient concentration and dissolved Oxygen. In addition, some strains isolated from oligographic media showed low affiliation with the sequences from the described species (less than 97 %), suggesting the presence of some novel taxa and the specific strains in antarctic euphotic layer. Therefore as it is revealed in this study, the bacterioplankton resources are rich in antarctic euphotic layer. Massive unknown microorganism deserve further studying and exploration as valuable resources．

Key words: Antarctic, Prydz Bay, bacteria, biodiversity

Wu Yuehong, Han Zhengbing, Zhang Xinqi, Zhou Yadong, Wu Min, Xu Xuewei. COMMUNITY COMPOSITION OF ANTARCTIC BACTERIOPLANKTON ISOLATED FROM THE PRYDZ BAY, ANTARCTICA[J]. ADVANCES IN POLAR SCIENCE, 2014, 26(2): 222-229.

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