Abstract:

One actinobacteria strain, R-527F, was isolated from the north off 73 ° N of the Arctic Ocean sediments. According to the morphological, physiological characteristics and its 16S rDNA analysis, it is the species within the genus of Streptomyces, the group of Globisporus, which is temporarily named as Streptomyces sp. R-527F. The anti-biology experiments based on amplification of biosynthetic genes and bacteria-inhibition model were carried out, the results showed that this strain has significant inhibition activity towards B. subtilis, the diameters of inhibition zones was 18.24 mm. Based on the variation of single factor experiments, the optimal fermentation condition of antibiotic substances biosynthetic was: fermentation media: starch 10 g, sucrose 10 g, soy bean powder 6 g, MgSO4 0.5 g, sea water 1000 ml; inoculum concentration 4%, initial pH 8.0, fermentation temperature 28 ℃, incubated for 6 days in 180 r/min of 40% media volume.

Key words: Antarctic, Actinomycete, Identification, Anti-bacteria activity