Chinese Journal of Polar Research

ADVANCES IN POLAR SCIENCE ›› 2011, Vol. 23 ›› Issue (4): 283-288.DOI: 10.3724/SP.J.1084.2011.00283

• Contents • Previous Articles     Next Articles

HIGH-LEVEL EXPRESSION OF A COLD-ADAPTIVE LIPASE GENE IN PICHIA PASTORIS

Wang Yongjie1,2，Shen Jihong1,2，Cong Bailin1,2，Lin Xuezheng1,2，Huang Xiaohang1,2   

  1. 1. First Institute of Oceanography, SOA
    2. Key Lab of Marine Bioactive Substances, SOA
  • Received:2011-02-28 Revised:2011-06-15 Online:2011-12-30 Published:2011-12-30

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Abstract:

A cold-adaptive lipase gene lip-948 from Antarctic psychrophilic bacteria was ligated into the expression vector pPIC9K, which was transformed into yeast GS115 subsequently. High-level express yeast was obtained . Lipase activity of the supernant reached 27.5 U/mL after 48 h fermentation, which is higher than most of other low-temperature bacteria lipase genes expressed in Pichia pastoris.

Key words: low-temperature lipase, pPIC9K, Pichia pastoris, High-level expression