DIVERSITY ANALYSIS OF FUNGI ISOLATED FROM NY-ÅLESUND, SVALBARD, AND SCREENING FOR BIOTRANSFORMATION OF GENTIOPICROSIDE

doi:10.3724/SP.J.1084.2012.00372

Abstract

Abstract: 65 strains of fungi isolated from the arctic high-latitude soil and marine sediments were investigated. Their phylogenetic diversity were analyzed by their 18S rDNA sequencing. Classification of 65 fungi strains based on 18S rDNA gene showed that these fungi represented 26 genera, of which the number of Penicillium was the most of 19 strains ,followed by Nectria and Articulospora ,with 6 strains respectively.The strains which can transform gentiopicroside were screened. β-glucosidase was chosen as marker enzyme to screen the positive strains. And 28 strains were tested to produce it. Among them, 9 strains were selected to transform gentiopicroside. And the crude extracts of them ware tested by TLC and HPLC. Ultimately strain C-5 was determined as the biotransformation strain. According to the morphological features and nucleic acid sequences analysis, strain C-5 was identified for the genus of Penicillium. It was tentatively named as Penicillium sp.C-5.

Key words: Arctic, fungi, diversity, β-glucosidase, biotransformation of gentiopicroside

Xu Dan, Ma Jifei, Miao Zhen, Li Huirong, Chen Bo. DIVERSITY ANALYSIS OF FUNGI ISOLATED FROM NY-ÅLESUND, SVALBARD, AND SCREENING FOR BIOTRANSFORMATION OF GENTIOPICROSIDE[J]. ADVANCES IN POLAR SCIENCE, 2012, 24(4): 372-383.

References

1 曾胤新，陈波.南极低温微生物研究及其应用前景.极地研究.1999，11（2）：143-150.

2 Ka-Lai Pang，Raymond K.K. Chow，Chi-Wong Chan et al. Diversity and physiology of marine lignicolous fungi in Arctic waters: a preliminary account. Polar Research.2011，30：5859.

3 Marit Frederikke， Markussen Bjorb?kmo，Tor Carlsen et al. High diversity of root associated fungi in both alpine and arctic Dryas octopetala. BMC Plant Biology. 2010，10：244.

4 Ka-Lai Pang，Michael W.L. Chiang，Lilian L.P. Vrijmoed. Havispora longyearbyenensis gen. et sp. nov.: an arctic marine fungus from Svalbard, Norway. Mycologia. 2008，100(2):291-5.

5 那广水，周传光，叶亮，等.北极微生物的分离及抗菌抗肿瘤活性筛选.水产科学.2008，27（8）：414-416.

6 郑洲，缪锦来，李光友.南极微生物中特殊酶类的研究进展.中国海洋药物杂志.2005，24（4）：49-55.

7 Puja Gawas-Sakhalkar，Shiv Mohan Singh. Fungal community associated with Arctic moss,Tetraplodon mimoides and its rhizosphere: bioprospecting for production of industrially useful enzymes.Current science.2011，100（11)：1701-1705.

8 Shiv M. Singh，Sanjay K. Singh，Lal S. Yadav et al. Filamentous Soil Fungi from Ny-?lesund, Spitsbergen, and Screening for Extracellular Enzymes. ARCTIC. 2012，Volume 65，issue 1 .

9 秦向东，刘吉开.2种北极高等真菌的化学成分分析.云南大学学报（自然科学版）.2012，34（2）：224-226.

10 陈皓文，高爱国.中国极地微生物学调查研究进展.极地研究.2005，17（4）：299-307.

11 N. Gunde-Cimerman，S. Sonjak，P. Zalar et al. Extremophilic fungi in arctic ice: a relationship between adaptation to low temperature and water activity. Physics and Chemistry of the Earth. 2003，28：1273–1278.

12 H. V?re，M. Vestberg，S. Eurola. Mycorrhiza and root-associated fungi in Spitsbergen.Mycorrhiza.1992，1：93-104.

13 宋欣，曲音波，袁晓华.一种将七叶苷用于高产β-葡萄糖苷酶产生菌的平板筛选的方法.中国.CN200710114837.2007.

14 James Borneman，Jack Hartin R．PCR primers that amplify fungal rRNA genes from environmental samples．Applied and environmental microbiology． 2000．4356～436.

15 Thompson J D，Gibson T J，Plewniak F.The Clustal X windows interface：flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Research.1997，24（4）：476-488.

16 Saitou N，Nei M.The neighbor-joining method：a new method for reconstructing phylogenetic tree.Mol Biol Evol.1987，4：406-425.

17 赵林果，孟鹏，李丽娟，等.利用七叶苷显色技术检验和判断β-葡萄糖苷酶的研究.食品与发酵工业.2008，34（12）：163-166.

18 Yi H，Yoon H I，Chun J. Sejongia Antarctica gen. nov. ，sp. nov. and Sejongia jeonii sp. nov. ，isolated from the Antarctic[J]. Int J Syst Evol Microbiol.2005，55（1）：409-416.

19 Wolfgang Dawid，Claudia A.Gallikowski，Peter Hirsch. 3.8 Psychrophilic myxobacteria from Antarctic soils. Polarforschung, 1988，58(2/3)：271?278.

20 Roseli Ocampo-Friedmann，E.Imre Friedmann.Biologically active substances produced by antarctic cryptoendolithic fungi.Antarct Journal US.1993，28(5)：252 ?254.

21 Hellio C，Bremer G，Pons A M，et al. Inhibition of the development of microorganisms (bacteria and fungi) by extracts of marine algae from Brittany，France[J]. Appl Microbiol Biotechnol.2000，54（4）：543-549.

22 B.C. Hitzfeld，C.S. Lampert，N .Spaeth, et al. Toxin production in cyanobacterial mats from ponds on the McMurdo ice shelf, Antarctica. Toxicon.2000，38(12)：1731-1748.

23 Yoshio T AKEDA，Toshiya MASUDA，Gisho HONDA ，et al. Secoiridoid Glycosides from Gentiana olivieri.Chem. Pharm. Bull.1999，47（9）：1338-1340

24 陈雷.龙胆苦苷衍生物合成及药理作用研究.西北大学博士学位论文.2008

25 雷军.钩藤后下机理及龙胆苦苷结构修饰的研究.西北大学硕士学位论文.2004

26 宋纯清，擂肖锋，米靖宇，等.龙胆苦苷的人肠内菌代谢产物研究.中国药学杂志.2000，35增刊：103-105

27 Adel I.EL-SEDAWY，Masao HATTORI，Kyoichi KOBASHI，et al. Metabolism of Gentiopicroside （Gentiopicrin）by Human Intestinal Baceria.Chem. Pharm. Bull.1989，37（9）：2435-2437

28 Dong Wang，Min Xu，Hong-Tao Zhu，et al. Biotransformation of gentiopicroside by asexual mycelia of Cordyceps sinensis.Bioorganic & Medicinal Chemistry Letters.2007，17：3195-3197

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