Abstract: The Ny-Ålesund Region (78°55′N, 11°56′E) is located on the western coast of Svalbard, which is the northernmost settlement in the Arctic. To date, the diversity and ecological distribution of macrofungi in this region have been poorly studied. In this study, we collected a total of 48 macrofungal samples in this region during July/August 2019. They were identified as 21 species belonging to one order, seven families, and 10 genera based on morphological characteristics and molecular sequence analysis. One species, Ar-rhenia rustica, was found for the first time in the Svalbard Islands. The floristic analysis showed that the macrofungal species in the region had obvious holarctic distributions as species with North-Temperate and Arctic distributions and North-Frigid distributions were the main geographical components. This study enriches the understanding of macrofungal diversity in the Ny-?lesund Region and provides basic data for studying the impact of climate change on fungal diversity in the Arctic tundra.

Key words: macrofungi, species diversity, molecular phylogenetics, floristic analysis