极地研究

极地研究 ›› 2009, Vol. 21 ›› Issue (1): 33-42.

• 研究论文 • 上一篇    下一篇

北极黄河站植物根际土壤放线菌分离培养及其鉴定

滕海波, 唐学玺, 肖慧, 俞勇, 李会荣   

  1. 中国海洋大学海洋生命学院; 国家海洋局极地科学重点实验室; 中国极地研究中心;
  • 出版日期:2009-03-30 发布日期:2009-03-30
  • 基金资助:

    中国极地战略研究基金资助。

ISOLATION AND IDENTIFICATION OF ACTINOBACTERIA FROM RHIZOSPHERIC SOIL IN THE ARCTIC YELLOW RIVER STATION

TENG Hai-Bei, TANG Hua-Xi, XIAO Hui, SHU Yong, LI Hui-Rong   

  1. 1 College of Marine Life Science; Ocean University of China; Qingdao 266003; China; 2 SOA key laboratory for polar science; Polar Research Institute of China; Shanghai 200136; China
  • Online:2009-03-30 Published:2009-03-30

PDF (PC)

2120

被引次数

58

摘要：

通过稀释涂布平板法,对北极黄河站(挪威新奥尔松地区)7种常见植物根际土壤样品进行放线菌的分离培养。经扩增性rDNA限制性酶切片段分析(ARDRA)及16S rDNA序列分析确定放线菌53株,分别属于8个属,除了优势菌链霉菌属(Streptomyces)和节杆菌属(Arthrobacter)外,还发现了红球菌(Rhodococcus)、糖丝菌(Saccharothrix)、拉塞氏杆菌(Rathayibacter)、微球菌(Micrococcus)、诺卡氏菌(Nocardia)、克里贝拉(Kribbella)等6种稀有菌属。

关键词: 北极, 根际土壤, 放线菌, 16S rDNA, 北极, 根际土壤, 放线菌, 16S rDNA

Abstract:

Using spread-plate technique,actinobacterial strains were isolated from rhizospheric soil of 7 plants collected in the Arctic Yellow River Station(Ny-Alesund,the Arctic). 53 actinobacteria were indentified by the analysis of ARDRA and 16S rDNA sequences and were shown to represent strains from 8 genera: Streptomyces and Arthrobacter which is predominant and rare actinobacteria Rhodococcus,Saccharothrix,Rathayibacter,Micrococcus,Nocardia,Kribbella.

Key words: the Arctic, rhizosphere, actinobacteria, 16S rDNA