极地研究

极地研究 ›› 2011, Vol. 23 ›› Issue (3): 189-195.DOI: 10.3724/SP.J.1084.2011.00189

• 研究论文 • 上一篇    下一篇

一株南极生境来源木霉菌株抗菌及诱导植物抗盐抗寒作用的初步研究

贾炜1,2,田黎1,2,陶晶晶1,史振平1,张久明1   

  1. 1. 青岛科技大学
    2. 国家海洋局第一海洋研究所
  • 收稿日期:2010-12-29 修回日期:2011-05-12 出版日期:2011-09-30 发布日期:2011-09-30
  • 通讯作者: 田黎
  • 基金资助:

    国家高技术”863”研究发展计划

PRELIMINARY STUDY ON ANTIBACTERIAL ACTIVITY OF A TRICHODERMA STRAIN FROM AN ANTARTIC MOSS HABITAT AND ITS SALT,COLD RESISTANCE ON PLANTS

JIA Wei1,2, TIAN Li1,2 ，TAO Jingjing 1,2, SHI Zhenping1, ZHANG Jiuming1   

  1. 1. Qingdao University of science and technology 2. The First Institute of Oceanography, SOA
  • Received:2010-12-29 Revised:2011-05-12 Online:2011-09-30 Published:2011-09-30
  • Contact: Li TIAN

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摘要：

由南极苔藓样品中分离到一株木霉菌株NTYM-0112，利用传统分类学与ITS序列及系统发育分析相结合方法对其进行分类鉴定，并对其抑植物病原菌活性、范围及对植物抗盐、抗寒的诱导作用进行了初步研究。结果表明，该菌株属于棘孢木霉（Trichoderma asperellum），对稻瘟霉菌(Pyrfcularia oryzae)、棉花黄萎菌（Verticillium dahliae）、小麦赤霉病菌（Fusarium graminearum）等7种常见植物病原菌有明显的抑制作用。番茄种子经该菌株处理后发芽率比对照提高了13.04%，番茄苗盐害指数降低了35.7%，成活率提高了34.4%，干重含水量提高了20.4%，叶片内丙二醛含量比对照明显减少；黄瓜幼苗经该菌株处理后，在0℃、5℃、10℃下电解质外渗率比对照分别下降了13.1%、20.9%、32.8%，可溶性糖外渗值分别下降了18.0 mg/g、28.6 mg/g、48.4 mg/g，证明该菌株对植物具有良好的抑菌和诱导抗逆作用。

关键词: 抗寒

Abstract:

Using traditional taxonomic methods, ITS sequences and phylogenetic analysis, Trichoderma strain NTYM-0112 isolated from an Antarctic moss habitat was identified as T. asperellum. A preliminary study was made of its antibacterial activities and range in inducing salt and cold resistance effects in plants. The antibacterial activities results revealed that strain NTYM-0112 showed obvious inhibition to seven common plant pathogens including Pyrfcularia oryzae, Verticillium dahlia and Fusarium graminearum. Salt resistance results indicated that the germination rate of tomato seeds increased 13.4% compared with the control after treatment with strain NTYM-0112; the salt injury index reduced significantly by 35.7%; and the survival rate and water content of tomato dry weight increased by 34.4% and 20.4%, respectively, after treatment with strain NTYM-0112. The MDA content of leaves was obviously reduced compared with the control. The cold resistance results showed that after treatment with strain NTYM-0112, the electrolyte leakage rate of cucumber seedlings at 0°C, 5°C and 10°C reduced by 13.1%, 20.9% and 32.8%, respectively, and the extravasation values of soluble sugar decreased by 18.0 mg/g, 28.6 mg/g and 48.4 mg/g, respectively. All of these results confirmed Antarctic habitat strain Trichoderma asperellum possessed preferable antibacterial activity and effect of inducing plant resistance to stress.

Key words: cold resistance