极地研究

极地研究 ›› 2011, Vol. 23 ›› Issue (3): 183-188.DOI: 10.3724/SP.J.1084.2011.00183

• 研究论文 • 上一篇    下一篇

10种极地微藻叶绿素荧光特征分析

光应芝1,张芳2,张前前1,蔡明红2,何剑锋2   

  1. 1.中国海洋大学化学化工学院
    2.国家海洋局极地科学重点研究室，中国极地研究中心
  • 收稿日期:2011-03-04 修回日期:2011-03-29 出版日期:2011-09-30 发布日期:2011-09-30
  • 通讯作者: 光应芝
  • 基金资助:

    国家自然科学基金

ANALYSIS OF CHLOROPHYLL FLUORESCENCE CHARACTERISTICS ON TEN KINDS OF POLAR MICROALGAE

Guang Yingzhi1,2，Zhang Fang2，Zhang Qianqian1，Cai Minghong2，He Jianfeng2   

  1. 1 College of Chemistry and Chemical Engineering，Ocean University of China

    2 Polar Research Institute of China，Key Labratory for Polar Science，SOA

  • Received:2011-03-04 Revised:2011-03-29 Online:2011-09-30 Published:2011-09-30
  • Contact: Ying-Zhi GUANG

PDF (PC)

1282

被引次数

14

摘要：

海洋浮游植物是水体环境中重要的初级生产者，其光合能力与浮游植物生产力密切相关，而这些均能在叶绿素荧光动力学上得以体现。利用调制叶绿素荧光仪(Water-PAM)对实验室培养的10种极地微藻的叶绿素荧光参数(Fv /Fo，Fv /Fm，NPQ)和快速光曲线拟合参数(Pm、α和Ik)进行测量和分析。结果表明：除CCMP2084外，其余9种微藻处均于正常生长状态。PSⅡ潜在活性值Fv/Fo大于1.0的藻共有8种，大小顺序分别为CCMP1056＞CCMP1204＞CCMP165＞CCMP2095＞CCMP1309＞CCMP445＞CCMP213＞CCMP2087。其中短孢角毛藻CCMP165、四棘藻CCMP213和海链藻CCMP1056三种藻的光保护能力较强(NPQ值分别大于2.0)。这三种藻对光照耐受能力强弱顺序为CCMP213＞CCMP1056＞CCMP165(比较三种藻的Ik值)。因此，四棘藻CCMP213和海链藻CCMP1056比短孢角毛藻CCMP165更适合在较强的光照强度下培养。

关键词: 快速光曲线

Abstract:

Marine phytoplanktons are important primary producer in aquatic environment and the productivity of them is related to its photosynthetic capacity. Chlorophyll fluorescence parameters (Fv /Fo, Fv /Fm, ΦPSⅡand NPQ) as well as the rapid light curve containing photosynthetic efficiency (a), maximal electron transport rate (Pm) and minimum saturating irradiance (Ik) of ten kinds of polar microalgae strains were measured by Water-PAM respectively. Nine kinds of strains except for CCMP2084 were in healthy physiological status. Fv /Fo of eight strains, whose values were higher than 1.0, showed activity sequence of PSⅡ as CCMP1056＞CCMP1204＞CCMP165＞CCMP2095＞CCMP1309＞CCMP445＞CCMP213＞CCMP2087. Besides, three strains including Chaetoceros brevis CCMP165, Attheya sp.CCMP213 and Thalassiosira sp CCMP1056, exhibit stronger photoprotection with the NPQ value higher than 2.0. Compared by Ik value, the sequence of light tolerance was CCMP213＞CCMP1056＞CCMP165. The strains of Attheya sp CCMP213 and Thalassiosira sp.CCMP1056 showed better adaptation to high irradiance than Chaetoceros brevis CCMP165.

Key words: Rapid Light Curve