极地研究

极地研究 ›› 2011, Vol. 23 ›› Issue (3): 196-204.DOI: 10.3724/SP.J.1084.2011.00196

• 研究论文 • 上一篇    下一篇

极地寡营养细菌AT52 (Alteromonas stellipolaris sp. nov.)超微结构、生长生理特性及液态富营养发酵培养条件优化

赵家路1,2,3,杨季芳1,2,陈福生3   

  1. 1.宁波市微生物与环境工程重点实验室
    2. 浙江万里学院生物与环境学院
    3.华中农业大学食品科技学院  
  • 收稿日期:2011-01-07 修回日期:2011-03-16 出版日期:2011-09-30 发布日期:2011-09-30
  • 通讯作者: 杨季芳
  • 基金资助:

    国家科技部国际合作重点资助项目;中德政府海洋双边合作项目;宁波市科技局资助项目

Ultrastructure, growth, physiological property, oligotrophic and nutritious liquid fermentation medium studies of Alteromonas stellipolaris sp. nov.

Zhao Jialu1,2,3 , Yang Jifang 1,2 , Chen Fusheng 3   

  1. 1.Municipal Key Laboratory of Microorganism and Environmental Engineering, Ningbo
    2. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo
    3.College of Food Science and Technology, Huazhong Agricultural University, Wuhan
  • Received:2011-01-07 Revised:2011-03-16 Online:2011-09-30 Published:2011-09-30

PDF (PC)

1384

被引次数

1

摘要：

寡营养细菌是一类能利用低浓度有机营养基质的细菌，通过研究一株分离自南极海洋的极地寡营养细菌AT52，发现富营养条件下AT52菌体细胞周身附肢，附肢长短不一，具鞭毛,菌体出芽生殖，由一层原生质膜包围。寡营养饥饿生存的菌体细胞相对较小，近球形，边缘不平滑，无鞭毛附肢等超微结构，细胞休眠无出芽生殖过程。实验中采用寡营养环境和富营养环境分别研究AT52菌的生长生理特性。优化得到该菌富营养条件下的最佳培养条件为：1L南极陈海水中加入蛋白胨5g，酵母粉1g，培养温度12℃，液体培养基的pH值为8.0，转速为180r/min，装液量为500ml三角瓶中装200ml，通氧量充分。

关键词: 最佳培养条件

Abstract:

Oligotrophic bacteria are a class can take advantage of low concentrations of organic nutrients matrix of bacteria, Oligotrophic bacteria AT52 is a strain isolated from the Antarctic polar ocean,its found AT52 cell have appendages, with flagella, cell budding , appendages of different lengths, surrounded by a layer of plasma membrane under the conditions in deep nutrient-rich. Oligotrophic bacterial cells to survive starvation is relatively small, nearly spherical, the edge is not smooth, without appendages such as flagella ultrastructure of cells as non-dormant buds of reproductive processes.This experiment studied the growth physiological characteristic of AT52,separately,in oligotrophic environment and rich nutrition environment.The optimal matrix: Poptone 5g,Yeast Extract 1g,the Antarctic seawater 1000mL.The optimal conditions: temperature 12℃,pH 8.0,rotation speed 180r/min, inoculation density 5％ and 200mL liquid medium joined into the 500mL Triangle bottle to ensure enough oxygen. AT52’s production cycle was shorted by thses conditions which provides the basis for industrial production.

Key words: optimal conditions