极地研究

极地研究 ›› 2012, Vol. 24 ›› Issue (1): 8-14.DOI: 10.3724/SP.J.1084.2012.00008

• 研究论文 • 上一篇    下一篇

北极海洋放线菌R-527F的抗菌活性筛选、鉴定及其抗菌物质合成条件研究

王毓舒1,2,曾倩1,2,俞勇2,陈波2   

  1. 1华东理工大学生物工程学院；2国家海洋局极地科学重点实验室，中国极地研究中心
  • 收稿日期:2011-05-09 修回日期:2011-05-17 出版日期:2012-03-30 发布日期:2012-03-30
  • 通讯作者: 陈波
  • 基金资助:

    中国极地科学战略研究基金;海洋公益性行业科研专项经费项目

Studies on the anti-biology activity , identification and fermentation condition of antibiotic substances of Streptomyces sp. R-527F isolated from Arctic marine sediment

  1. 1East China University of  Scinence and Technology；2Polar Research Institute of China
  • Received:2011-05-09 Revised:2011-05-17 Online:2012-03-30 Published:2012-03-30

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摘要：

对分离自北冰洋７３°Ｎ以北海域沉积物中的一株放线菌Ｒ５２７Ｆ进行分子鉴定及生物活性研究。通过对菌株的形态特征、培养性状、生理生化特征以及１６ＳｒＤＮＡ序列进行综合分析，确定该株菌为链霉菌属，暂定名为Ｓｔｒｅｐｔｏｍｙｃｅｓ ｓｐ．Ｒ５２７Ｆ。其含有聚酮类Ⅰ型（ＰＫＳⅠ）和非核糖体含硫多肽类（ｎｏｎｒｉｂｏｓｏｍａｌｐｅｐｔｉｄｅｓｙｎｔｈｅｔａｓｅ，ＮＲＰＳ）化合物的合成基因。对其抗菌活性及活性物质发酵条件进行了研究，实验结果表明，该菌株所产生的抗菌物质对枯草芽孢杆菌具有显著抑制效果，抑菌圈直径达到１８．２ｍｍ。适合其抗菌物质的发酵条件：种子液接种量为４％，培养基初始ｐＨ为８．０，培养温度为２８℃，培养时间为６ｄ，摇床转速为１８０ｒ?ｍｉｎ－１，装液量为４０％，为该菌株抗菌物质的进一步研究奠定发酵条件参数基础。

关键词: 北极, 放线菌, 鉴定, 抗菌活性

Abstract:

One actinobacteria strain, R-527F, was isolated from the north off 73 ° N of the Arctic Ocean sediments. According to the morphological, physiological characteristics and its 16S rDNA analysis, it is the species within the genus of Streptomyces, the group of Globisporus, which is temporarily named as Streptomyces sp. R-527F. The anti-biology experiments based on amplification of biosynthetic genes and bacteria-inhibition model were carried out, the results showed that this strain has significant inhibition activity towards B. subtilis, the diameters of inhibition zones was 18.24 mm. Based on the variation of single factor experiments, the optimal fermentation condition of antibiotic substances biosynthetic was: fermentation media: starch 10 g, sucrose 10 g, soy bean powder 6 g, MgSO4 0.5 g, sea water 1000 ml; inoculum concentration 4%, initial pH 8.0, fermentation temperature 28 ℃, incubated for 6 days in 180 r/min of 40% media volume.

Key words: Antarctic, Actinomycete, Identification, Anti-bacteria activity