Chinese Journal of Polar Research

ADVANCES IN POLAR SCIENCE ›› 2006, Vol. 17 ›› Issue (1-English): 55-60.

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Screening on DHA-producing Antarctic bacteria N-6 and its cultural conditions

 Zhang Botao1, Miao Jin-lai2, Hui Hanxing3 and Wang Qing3   

  1. 1 Life Sciences and Technology College, Ocean University of China, Qingdao 266003, China;
    2 Key Laboratory of Marine Bio-active Substances, State Ocean Administration, Qingdao 266061, China;
    3 Shandong Institute and Laboratory of Geological Sciences, Jinan 250013, China
  • Online:1956-03-30 Published:1956-03-30
  • Contact: Zhang Botao

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Abstract:

In Antarctic, the geography and climate differs from those in other places, and the bacteria there have adapted well to the environment there. Two hundred strains of bacteria were isolated from the sea ice in Antarctica. The bacteria were screened for DHA by means of GC, with fish oil as the standard. Seven strains containing DHA or EPA were obtained, among which the strain of No. N-6 was outstanding. And the component of DHA was identified by GC-MS. The relative content of DHA in N-6 was 8.72%, and total lipids in dry bacteria was 22.54%. The effects of some factors, including temperature, salinity and pH value, on the growth and DHA-content of strain N-6 were studied. The results show that the DHA-content is relatively high when in low temperature and high pH, and the bacterium is psychrophilic, alkalophilic.

Key words: Antarctic, bacteria, GC-MS, unsaturated fatty acids