Abstract:

A trehalose synthase-producing bacterial strain BSwl0041 isolated from Antarctic seawater was reported in the present paper. Morphology observation showed it to be a motile gram-negative bacterium with rod shape, polar flagella. The traditional physiological and biochemical methods, together with BIOLOG GN2 system were applied in the bacterial classification. Results showed that strain BSw10041 was similar to Pseudomonas putida in most of the pheno-types. According to the BIOLOG GN2 results, the BSwl0041 is 84. 8% similarity to Pseudomonas putida. To investigate the phylogenetic position of this bacterial strain, a 1658bp of 16S rRNA gene of strain BSw10041 was sequenced and compared with that of related strains. Phylogenetic tree was constructed based on the genetic distance analysis, which revealed that strain BSwl0041 is highest similar to P. putida ( GenBank accession number Z76667). The similarity value between BSwl0041 and P. putida ( Z76667 ) is 99. 9%. Based on the results obtained , strain BSw 10041 was identified as Pseudomonas putida and named for Pseudomonas putida BSw10041.