Abstract:

Un-cultivation and cultivation methods based on 16S rDNA gene sequences were applied to determine the bacterial community composition and diversity in soil from Deception Island, Antarctic. The 16S rDNA clone library analysis revealed that the cloned sequences were assigned to 39 operational taxonomic units (OTUs) in three phyla, namely Actinobacteria, Proteobacteria, and Bacteroidetes. The dominant phyla Actinobacteria and Proteobacteria accounted for 65.25% and 28.81% of the total clones, respectively. The dominant genera of Actinobacteria were Sporichthya, Nocardioides, Conexibacter, Gaiella, and Arthrobacter. The dominant genera of Proteobacteria were Sphingomonas, Thiobacillus, Stenotrophomona, and Thermomonas. A total of 57 isolates were obtained and were assigned to 13 OTUs in four phyla, namely Actinobacteria, Proteobacteria, Bacteroidetes, and Firmicutes. The dominant Actinobacteria and Proteobacteria accounted for 57.89% and 31.58% of the isolates, respectively. The dominant genera were Arthrobacter and Leifsonia from Actinobacteria and Pseudomonas from Proteobacteria. This study provides important insights into soil bacterial diversity and beneficial microbial resources from Deception Island, Antarctic.

Key words: Antarctic, Deception Island, Bacterial diversity, 16S rDNA clone library, Cultivation method