Abstract:

Oligotrophic bacteria are a class can take advantage of low concentrations of organic nutrients matrix of bacteria, Oligotrophic bacteria AT52 is a strain isolated from the Antarctic polar ocean,its found AT52 cell have appendages, with flagella, cell budding , appendages of different lengths, surrounded by a layer of plasma membrane under the conditions in deep nutrient-rich. Oligotrophic bacterial cells to survive starvation is relatively small, nearly spherical, the edge is not smooth, without appendages such as flagella ultrastructure of cells as non-dormant buds of reproductive processes.This experiment studied the growth physiological characteristic of AT52,separately,in oligotrophic environment and rich nutrition environment.The optimal matrix: Poptone 5g,Yeast Extract 1g,the Antarctic seawater 1000mL.The optimal conditions: temperature 12℃,pH 8.0,rotation speed 180r/min, inoculation density 5％ and 200mL liquid medium joined into the 500mL Triangle bottle to ensure enough oxygen. AT52’s production cycle was shorted by thses conditions which provides the basis for industrial production.

Key words: optimal conditions