极地研究

极地研究 ›› 2012, Vol. 24 ›› Issue (4): 372-383.DOI: 10.3724/SP.J.1084.2012.00372

• 研究论文 • 上一篇    下一篇

北极Ny-Ålesund地区真菌多样性分析及龙胆苦苷转化菌株的筛选

许丹1,2  马吉飞2  苗祯2  李会荣2  陈波2   

  1.  
    1华东理工大学生物工程学院，上海 200237；
    2国家海洋局极地科学重点实验室，中国极地研究中心，上海 200136
  • 收稿日期:2012-05-10 修回日期:2012-05-16 出版日期:2012-12-30 发布日期:2012-12-30
  • 通讯作者: 陈波

DIVERSITY ANALYSIS OF FUNGI ISOLATED FROM NY-ÅLESUND, SVALBARD, AND SCREENING FOR BIOTRANSFORMATION OF GENTIOPICROSIDE

Xu Dan1,2, Ma Jifei2, Miao Zhen2, Li Huirong2, Chen Bo2   

  1. 1School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China; 2SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China
  • Received:2012-05-10 Revised:2012-05-16 Online:2012-12-30 Published:2012-12-30

PDF (PC)

1325

摘要： 采用多种培养基对北极高纬地区（土壤及海洋沉积物）真菌进行分离纯化，共得真菌65株。通过分析菌株的18S rDNA序列研究了它们的系统发育多样性，18S rDNA序列分析表明，65株真菌分属25个属，其中数量上位居第一的是青霉属（Penicillium），有菌株20株，其次为丛赤壳属（Nectria）和节枝孢属（Articulospora），各有菌株6株。同时对这65株真菌开展转化龙胆苦苷阳性菌株的筛选。以β-葡萄糖苷酶作为筛选转化菌株的标志酶于65株真菌中筛选到阳性菌株28株，其中产酶能力强的9株菌作为转化实验的备选菌株，以龙胆苦苷为底物进行转化，将转化粗提物进行TLC及HPLC分析，最终确定将C-5作为转化龙胆苦苷的菌株。根据菌株形态特征和核酸序列分析结果，初步鉴定菌株C-5为青霉属真菌。暂定名为Penicillium sp.C-5。

关键词: 北极, 真菌, 多样性, β-葡萄糖苷酶, 龙胆苦苷生物转化

Abstract: 65 strains of fungi isolated from the arctic high-latitude soil and marine sediments were investigated. Their phylogenetic diversity were analyzed by their 18S rDNA sequencing. Classification of 65 fungi strains based on 18S rDNA gene showed that these fungi represented 26 genera, of which the number of Penicillium was the most of 19 strains ,followed by Nectria and Articulospora ,with 6 strains respectively.The strains which can transform gentiopicroside were screened. β-glucosidase was chosen as marker enzyme to screen the positive strains. And 28 strains were tested to produce it. Among them, 9 strains were selected to transform gentiopicroside. And the crude extracts of them ware tested by TLC and HPLC. Ultimately strain C-5 was determined as the biotransformation strain. According to the morphological features and nucleic acid sequences analysis, strain C-5 was identified for the genus of Penicillium. It was tentatively named as Penicillium sp.C-5.

Key words: Arctic, fungi, diversity, β-glucosidase, biotransformation of gentiopicroside