南极阿德雷岛不同基底中真菌的分离培养及初步鉴定

doi:10.3724/SP.J.1084.2013.00071

极地研究 ›› 2013, Vol. 25 ›› Issue (1): 71-77.DOI: 10.3724/SP.J.1084.2013.00071

南极阿德雷岛不同基底中真菌的分离培养及初步鉴定

靳永轩^1,2 丛柏林^1,2王能飞^1,2 刘胜浩^1,2 沈继红^1,2 黄晓航^1,2

¹国家海洋局第一海洋研究所，山东青岛 266061； ²国家海洋局海洋生物活性物质重点实验室，山东青岛 266061

收稿日期:2012-09-25 修回日期:2012-11-08 出版日期:2013-03-30 发布日期:2013-03-30
通讯作者: 丛柏林
基金资助:
极地专项

ISOLATION AND PRELIMINARY IDENTIFICATION OF MICROFUNGI IN SAMPLES COLLECTED FROM ARDLEY ISLAND, ANTARCTICA

Jin Yongxuan^{1, 2}, Cong Bailin^{1, 2}, Wang Nengfei^{1, 2}, Liu Shenghao^{1, 2}, Shen Jihong^{1, 2}, Huang Xiaohang^{1, 2}

¹Key Laboratory of Marine Bioactive Substances, SOA, Qingdao 266061, China;
²The First Institute of Oceanography, SOA, Qingdao 266061, China

Received:2012-09-25 Revised:2012-11-08 Online:2013-03-30 Published:2013-03-30
Contact: Cong Bailin

摘要/Abstract

摘要： 为了探索南极真菌的多样性，进一步揭示其生态学角色和筛选有特定酶活性菌株，本研究对中国第27次南极科学考察采集自阿德雷岛的6份样品进行了真菌的分离培养及其胞外酶活性测定。从6份样品中共分离得到168株真菌，其中大型海藻腐烂物样品中分离到的真菌种类和数量最多，占所分离菌株总数的34.6%；对选取的15株代表菌株进行了真菌ITS1-5.8S-ITS2序列鉴定，结果表明它们分属于4个纲，8个属，其中青霉属5株，假散囊菌属、地丝菌属和枝孢属各2株，生赤壳属、曲霉属、出芽短梗霉属和unclassified Onygenales各 1株，该结果表明南极地区具有丰富的真菌物种多样性，其优势类群青霉菌。胞外酶测定显示产淀粉酶、蛋白酶和纤维素酶产生菌分别占供试菌株的64.6%、45.1%、14.6%，产纤维素酶的菌株可能在南极物质能量循环中扮演着重要角色。

关键词: 南极, 阿德雷岛, 真菌多样性, 酶活

Abstract: In order to explore the diversity of Antarctic fungi and determine their role in polar ecological system, micro-fungi were isolated from substrate samples collected at the Ardley Island during Chinese 27th Antarctic Scientific Expedition. Our results showed that a total of 168 fungi strains were isolated from six Antarctic samples, and the fungi with more variety and higher amount were those isolated from rotten macroalgae, accounting for 34.6% of the total isolates. The Internal Transcribed Spacer (ITS) rDNA of 15 representative strains were sequenced and the phylogenetic tree was constructed using Mega software. Results showed that these fungi belong to four classes, eight genera, and they are Penicilliumm (5 strains), Pseudeurotium (2 strains), Geomyces (2 stains), Cladosporium (2 stains), Bionectria (1 strain), Aspergillus(1 strain), Aureobasidium(1 strain) and unclassified Onygenales (1 strain). These results suggested that the Antarctic region may have abundant species diversities of fungi. Furthermore, the analysis on extracellular enzymes revealed that 64.6%, 45.1%, 14.6% of the total tested strains could produce amylase, protease and cellulase respectively. These enzyme secretion fungi may play an important role in the energy flow and material circulation in Antarctic continent.

Key words: Antarctica, Ardley Island, fungal diversity, enzyme activity

靳永轩丛柏林王能飞刘胜浩沈继红黄晓航. 南极阿德雷岛不同基底中真菌的分离培养及初步鉴定[J]. 极地研究, 2013, 25(1): 71-77.

Jin Yongxuan, Cong Bailin, Wang Nengfei, Liu Shenghao, Shen Jihong, Huang Xiaohang. ISOLATION AND PRELIMINARY IDENTIFICATION OF MICROFUNGI IN SAMPLES COLLECTED FROM ARDLEY ISLAND, ANTARCTICA[J]. ADVANCES IN POLAR SCIENCE, 2013, 25(1): 71-77.

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南极阿德雷岛不同基底中真菌的分离培养及初步鉴定

ISOLATION AND PRELIMINARY IDENTIFICATION OF MICROFUNGI IN SAMPLES COLLECTED FROM ARDLEY ISLAND, ANTARCTICA

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