南极磷虾中的氟对大鼠软组织的毒性评估

doi:10.3724/SP.J.1084.2013.00078

极地研究 ›› 2013, Vol. 25 ›› Issue (1): 78-83.DOI: 10.3724/SP.J.1084.2013.00078

南极磷虾中的氟对大鼠软组织的毒性评估

张玲^1,2 陆晓奇^1,2 王张民^1,2 秦立强³ 袁林喜² 尹雪斌^1,2

¹中国科学技术大学地球和空间科学学院，安徽合肥 230026；

²中国科学技术大学苏州研究院生态安全与人体健康重点实验室，江苏苏州 215123；
³苏州大学医学部放射医学与公共卫生学院营养与食品卫生学教研室，江苏苏州 215123

收稿日期:2012-10-17 修回日期:2012-12-06 出版日期:2013-03-30 发布日期:2013-03-30
通讯作者: 尹雪斌
基金资助:
国家海洋局极地科学重点实验室开放研究基金

EVALUATION OF THE EFFECT OF FLUORINE TOXICITY IN ANTARCTIC KRILL ON SOFT TISSUE IN RATS

Zhang Ling^1,2, Lu Xiaoqi^1,2, Wang Zhangmin^1,2, Qin Liqiang³, Yuan Linxi¹, Yin Xuebin^1,2

¹School of Earth and Space Science,University of Science and Technology of China, Hefei 230026, China;

²Suzhou Key Lab for Eco-safety and Human Health, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou 215123, China; ³Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, 215123, China

Received:2012-10-17 Revised:2012-12-06 Online:2013-03-30 Published:2013-03-30

摘要/Abstract

摘要： 应用动物实验模型，通过喂食Wistar大鼠南极磷虾，并设置对照组和相同氟剂量的氟化钠组，来评估、比较磷虾中氟的毒性。染毒结束取大鼠组织，进行苏木精和伊红（H-E）染色，观察切片病理变化，结果显示：对照组、磷虾组、氟化钠组大鼠体重及脏器系数没有显著性差异；对照组大鼠肝脏、肾脏、脾脏、脑组织未出现病变，磷虾组大鼠这四种组织均表现出少量病变，氟化钠组大鼠这四种组织的病变更加显著，反映了磷虾氟的毒性确实相对较低；对照组、磷虾组、氟化钠组的睾丸组织也没有发现病变。这些结果表明尽管南极磷虾氟的毒性较氟化钠低，但直接大量食用南极磷虾仍会对大鼠的软组织有一定的毒害作用，南极磷虾在开发作为食物时需要进行脱壳等脱氟处理。

关键词: 南极磷虾, 氟, 动物实验, H-E染色, 病理

Abstract: The toxicity of fluorine (F) in Antarctic krill via animal experiment was evaluated. There were three groups in this experiment: the control group, the krill treatment group (150 mg?kg-1 F), the NaF treatment group (150 mg?kg-1 F). After three months, the rats were dissected and the liver, kidney, spleen, brain and testis samples were collected. Morphological change of these sample cells was assessed by H-E staining. There was no significant difference of weight and viscera coefficient among three groups. Except for testis, the other four soft tissues samples in the krill treatment group display some pathological changes, although the pathological changes in krill treatment group were fewer than that in NaF treatment group. These results revealed that the toxicity of equal quality F in Antarctic krill was lower than that in NaF, but it was still toxic for rats to consume the krill excessively. This study provides the direct scientific evidences for exploring further human consumption of krill.

Key words: Antarctic krill, fluorine, animal experiment, HE staining, pathological changes

张玲陆晓奇王张民秦立强袁林喜尹雪斌 . 南极磷虾中的氟对大鼠软组织的毒性评估[J]. 极地研究, 2013, 25(1): 78-83.

Zhang Ling, Lu Xiaoqi, Wang Zhangmin, Qin Liqiang, Yuan Linxi, Yin Xuebin. EVALUATION OF THE EFFECT OF FLUORINE TOXICITY IN ANTARCTIC KRILL ON SOFT TISSUE IN RATS[J]. ADVANCES IN POLAR SCIENCE, 2013, 25(1): 78-83.

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南极磷虾中的氟对大鼠软组织的毒性评估

EVALUATION OF THE EFFECT OF FLUORINE TOXICITY IN ANTARCTIC KRILL ON SOFT TISSUE IN RATS

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