极地研究

极地研究 ›› 2011, Vol. 22 ›› Issue (1-English): 25-34.DOI: 10.3724/SP.J.1085.2011.00025

• 研究论文 • 上一篇    下一篇

Relationships between two Pseudoalteromonas strains isolated from the Canada Basin and the Southern Ocean using a polyphasic approach

Zeng YinXin1, 2* & Zheng TianLing2   

  1. 1 Polar Research Institute of China, Key Laboratory for Polar Science of State Oceanic Administration, Shanghai 200136, China;
    2 Xiamen University, School of Life Sciences, Institute of Applied and Environmental Microbiology, Xiamen 361005, China
  • 收稿日期:2010-09-10 修回日期:2010-10-08 出版日期:1961-03-30 发布日期:1961-03-30
  • 通讯作者: Zeng YinXin

Relationships between two Pseudoalteromonas strains isolated from the Canada Basin and the Southern Ocean using a polyphasic approach

Zeng YinXin1, 2* & Zheng TianLing2   

  1. 1 Polar Research Institute of China, Key Laboratory for Polar Science of State Oceanic Administration, Shanghai 200136, China;
    2 Xiamen University, School of Life Sciences, Institute of Applied and Environmental Microbiology, Xiamen 361005, China
  • Received:2010-09-10 Revised:2010-10-08 Online:1961-03-30 Published:1961-03-30
  • Contact: Zeng YinXin

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摘要：

The evolutionary relationships and taxonomic position of two marine planktonic bacterial strains BSw20211 and BSw10014, isolated from the Canada Basin and from the Southern Ocean, respectively, were determined using a polyphasic taxonomic approach. There was a close phylogenetic relationship between the two strains and most phenotypic properties were shared. Nonetheless, they were found to belong to different species of the genus Pseudoalteromonas on the basis of genotypic analyses. Findings were consistent with the suggestion that gyrB gene sequence comparison and DNA-DNA relatedness might better define phylogenetic relationships of bacteria at the species level. However, a cut-off value of 90% gyrB gene sequence similarity was not reliable for the differentiation of species within the genus Pseudoalteromonas.

关键词: Pseudoalteromonas, phylogenetic relationship, 16S rRNA, gyrB, DNA relatedness

Abstract:

The evolutionary relationships and taxonomic position of two marine planktonic bacterial strains BSw20211 and BSw10014, isolated from the Canada Basin and from the Southern Ocean, respectively, were determined using a polyphasic taxonomic approach. There was a close phylogenetic relationship between the two strains and most phenotypic properties were shared. Nonetheless, they were found to belong to different species of the genus Pseudoalteromonas on the basis of genotypic analyses. Findings were consistent with the suggestion that gyrB gene sequence comparison and DNA-DNA relatedness might better define phylogenetic relationships of bacteria at the species level. However, a cut-off value of 90% gyrB gene sequence similarity was not reliable for the differentiation of species within the genus Pseudoalteromonas.

Key words: Pseudoalteromonas, phylogenetic relationship, 16S rRNA, gyrB, DNA relatedness