极地研究

极地研究 ›› 2014, Vol. 26 ›› Issue (2): 186-192.DOI: 10.13679/j.jdyj.2014.2.186

• 研究论文 • 上一篇    下一篇

南极长城站空气微生物的分子生态学分析

夏晓敏1,2 汪建君3 陈立奇3 颜金培3 张锐1,2     

  1. 1厦门大学近海海洋环境科学国家重点实验室，福建 厦门 361005;
    2    厦门大学海洋微型生物与地球圈层研究所，福建 厦门 361005;
    3    国家海洋局第三海洋研究所，海洋-大气化学与全球变化重点实验室，福建 厦门 361005
  • 收稿日期:2013-02-04 修回日期:2013-04-17 出版日期:2014-06-30 发布日期:2014-06-30
  • 通讯作者: 张锐
  • 基金资助:

    自然科学基金;国家海洋局海洋—大气化学与全球变化重点实验室开放基金;厦门大学基础创新科研基金

BIODIVERSITY OF AIRBORNE BACTERIA AT THE GREAT WALL STATION, ANTARCTICA

Xia Xiaomin1,2,  Wang Jianjun3,  Chen liqi3,  Yan Jinpei3,  Zhang Rui1,2   

  1. 1State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China;
    2Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen 361005, China;
    3Key Laboratory of Global Change and Marine-Atmospheric Chemistry, State Oceanic Administration, Xiamen 361005, China
  • Received:2013-02-04 Revised:2013-04-17 Online:2014-06-30 Published:2014-06-30

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被引次数

2

摘要： 微生物是气溶胶的重要组成部分。本研究通过收集南极长城站附近气溶胶，构建气溶胶中空气微生物16S rRNA基因文库，发现南极空气微生物具有很高的多样性，93个克隆子包含了53个OTU(operational taxonomic unit)，Shannon指数为3.58，Simpson指数为0.04。南极长城站空气微生物的主要细菌类群为Actinobacteria、 Cyanobacteria/Chloroplasts、Bacteroidetes、Firmicutes、Planctomycetes、Proteobacteria及Verrucomicrobia等7个门，Proteobacteria门的细菌是优势类群，占到了长城站空气微生物细菌克隆文库的65.6%。克隆文库中海源微生物序列及陆源微生物序列各占到了30.1%，说明长城站附近具有较强的海陆大气交换。我们发现有15个克隆子序列与人类活动相关，占总克隆子数的16.1%，说明人类活动已经影响到了长城站的空气微生物群落结构。

关键词: 南极空气微生物, 微生物群落结构, 16S rRNA基因文库

Abstract: Bacteria are an important component of the aerosol, which is closely related with the human beings. This study analyzed the community structure of air-borne bacteria at The Great Wall Station, Antarctica, based on 16S rRNA gene clone library analysis. High diversity of air-borne bacteria was observed in the aerosol samples collected at The Great Wall Station. Ninety three sequences from the clone library can be classified into 53 OTUs (operation taxonomic unit) based on 97% sequence similarity. Shannon and Simpson index of the clone library are 3.58 and 0.04, respectively. Phylogenetic analysis showed the sequences can be affiliated into seven phylum: Actinobacteria、Cyanobacteria/Chloroplast、Bacteroidetes、Firmicutes、Planctomycetes、Proteobacteria and Verrucomicrobia. Proteobacterial sequences accounted for 65.6% of the clone library. There were same amount of sequences (30.1%) seemed to be derived from marine and terrestrial environments, suggesting a strong atmosphere exchange of the land and sea near The Great Wall Station. In addition, there were 15 sequences related with human activities, which indicated that human activities had already affected the airborne microbial community structure of The Great Wall Station.

Key words: Antarctica aerosol, bacteria community structure, 16S rRNA gene clone library