极地研究

极地研究 ›› 2010, Vol. 21 ›› Issue (1-English): 81-90.DOI: 10.3724/SP.J.1085.2010.00081

• 研究论文 • 上一篇    下一篇

Effect of high salinity on cell growth and protein production of Antarctic ice microalgae Chlamydomonas sp. ICE-L

Zheng Zhou1, Miao Jinlai1, Kan Guangfeng2, Jin Qing3, Ding Yu4, Liu Fangming1 and Wang Shouqiang1   

  1. 1 Key Laboratory of Marine Bio-active Substances, SOA, Qingdao 266061, China;
    2 College of Oceanology, Weihai Branch of Harbin University of Technology, Weihai 264209, China;
    3 College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, China;
    4 Ocean University of Guangdong, Zhanjiang 524025, China
  • 出版日期:1960-03-30 发布日期:1960-03-30
  • 通讯作者: Zheng Zhou

Effect of high salinity on cell growth and protein production of Antarctic ice microalgae Chlamydomonas sp. ICE-L

Zheng Zhou1, Miao Jinlai1, Kan Guangfeng2, Jin Qing3, Ding Yu4, Liu Fangming1 and Wang Shouqiang1   

  1. 1 Key Laboratory of Marine Bio-active Substances, SOA, Qingdao 266061, China;
    2 College of Oceanology, Weihai Branch of Harbin University of Technology, Weihai 264209, China;
    3 College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, China;
    4 Ocean University of Guangdong, Zhanjiang 524025, China
  • Online:1960-03-30 Published:1960-03-30
  • Contact: Zheng Zhou

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摘要：

Antarctic ice microalgae Chlamydomonas sp. ICE-L can survive and thrive in Antarctic sea ice. In this study, Chlamydomonas sp. ICE-L could survive at the salinity of 132‰ NaCl. SDS-PAGE showed that the density of 2 bands (26 and 36 kD) decreased obviously at the salinity of 99‰ NaCl compared to at the salinity of 33‰ NaCl. The soluble proteins in Chlamydomonas sp. ICE-L grown under salinity of 33‰ and 99‰ NaCl were compared by 2-D gel electrophoresis. After shocking with high salinity, 8 protein spots were found to disappear, and the density of 28 protein spots decreased. Inaddition, 19 protein spots were enhanced or induced, including one new peptide (51kD). The changes of proteins might be correlated with there sistance for Chlamydomonas sp. ICE-L to high salinity.

关键词: Antarctic ice alga, Chlamydomonas sp. ICE-L, soluble proteins, two-dimensional polyacrylamide , gel electrophoresis gel electrophoresis (2-DPAGE) analysis, high-salinity press

Abstract:

Antarctic ice microalgae Chlamydomonas sp. ICE-L can survive and thrive in Antarctic sea ice. In this study, Chlamydomonas sp. ICE-L could survive at the salinity of 132‰ NaCl. SDS-PAGE showed that the density of 2 bands (26 and 36 kD) decreased obviously at the salinity of 99‰ NaCl compared to at the salinity of 33‰ NaCl. The soluble proteins in Chlamydomonas sp. ICE-L grown under salinity of 33‰ and 99‰ NaCl were compared by 2-D gel electrophoresis. After shocking with high salinity, 8 protein spots were found to disappear, and the density of 28 protein spots decreased. Inaddition, 19 protein spots were enhanced or induced, including one new peptide (51kD). The changes of proteins might be correlated with there sistance for Chlamydomonas sp. ICE-L to high salinity.

Key words: Antarctic ice alga, Chlamydomonas sp. ICE-L, soluble proteins, two-dimensional polyacrylamide gel electrophoresis gel electrophoresis (2-DPAGE) analysis, high-salinity press