极地研究

极地研究 ›› 2007, Vol. 18 ›› Issue (1-English): 63-72.

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Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp. NJ298 in low temperature

 Tang Haitian1,2, Zheng Zhou2, Miao Jinlai2, Liu Junling2 and Kan Guangfeng2   

  1. 1 College of Life Sciences, Ludong University, Yantai 264025, China;
    2 Key Laboratory of Marine Bio-active Substances, First Institute of Oceanorgaphy, SOA, Qingdao 266061, China
  • 出版日期:1957-03-30 发布日期:1957-03-30
  • 通讯作者: Tang Haitian

Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp. NJ298 in low temperature

Tang Haitian1,2, Zheng Zhou2, Miao Jinlai2, Liu Junling2 and Kan Guangfeng2   

  1. 1 College of Life Sciences, Ludong University, Yantai 264025, China;
    2 Key Laboratory of Marine Bio-active Substances, First Institute of Oceanorgaphy, SOA, Qingdao 266061, China
  • Online:1957-03-30 Published:1957-03-30
  • Contact: Tang Haitian

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摘要：

The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at-3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at-3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18:1 and C18:2) and shorter chain saturated fatty acid (C10:0) increased along with the decrease of the culture temperature from 8 ℃ to-3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at-3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at-3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature.

关键词: psychrophile yeast, plasmamembrane fluidity, fatty acid, protein, carotenoids, cold-adaptation

Abstract:

The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at-3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at-3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18:1 and C18:2) and shorter chain saturated fatty acid (C10:0) increased along with the decrease of the culture temperature from 8 ℃ to-3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at-3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at-3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature.

Key words: psychrophile yeast, plasmamembrane fluidity, fatty acid, protein, carotenoids, cold-adaptation