极地研究

极地研究 ›› 2007, Vol. 19 ›› Issue (4): 255-265.

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产低温超氧化物歧化酶南极菌株的筛选、鉴定及其部分酶学性质

郑洲;缪锦来;   

  • 出版日期:2007-12-30 发布日期:2007-12-30

SCREENING AND CLASSIFICATION FOR LOW-TEMPERATURE SUPEROXIDE DISMUTASE-PRODUCING ANTARCTIC BACTERIA AND CHARACTERISTICS OF THE SUPEROXIDE DISMUTASES

  • Online:2007-12-30 Published:2007-12-30

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1769

被引次数

6

摘要：

从421株南极细菌中筛选出产超氧化物歧化酶南极细菌NJ062、NJ379、NJ522和NJ548。它们的最适生长温度在10—15℃之间,在0℃均能生长,属于嗜冷菌。4株南极嗜冷菌均含有一种类型的SOD同工酶。菌株NJ062的超氧化物歧化酶为Mn-SOD;菌株NJ379,NJ522和NJ548的超氧化物歧化酶均为Fe-SOD。它们所产的超氧化物歧化酶最适酶活温度大约在40—50℃,在0℃仍有较高的活性,为最高活性的20%—35%,热稳定性较差,属低温酶。通过16S rDNA序列对4株产低温超氧化物歧化酶的南极嗜冷菌NJ062、NJ379、NJ522和NJ548进行鉴定。结果表明,它们均属于细菌域(Bacteria)中的紫细菌类群(Proteobacteria)中的γ-亚群。其中菌株NJ062属于假交替单胞菌属(Pseudoalteromonassp.);NJ379属于希瓦氏菌属(Shewanellasp.);NJ522和NJ548属于海单胞菌属(Marinomonassp.)。

关键词: 南极;细菌;低温酶;超氧化物歧化酶;筛选;鉴定

Abstract:

Four SOD-producting bacteria NJ062,NJ379,NJ522 and NJ548 were selected from 421 Antarctic bacteria.They belonged to psychrophile for their optimum growth temperatures were about 10—15℃ and they could grow at 0 ℃ and they only contained one kind of SOD isoenzymes.The SOD of NJ062 was Mn-SOD,while the SODs of other three bacteria were Fe-SOD.The four SOD's activity at 0 ℃ was nearly 20%—35% of the maximum activity,and their maximal activity temperature was 40—50 ℃.In addition,the four SODs were unstable to higher temperature indicating that they were cold-active enzymes.Four Antarctic psychrophile NJ062,NJ379,NJ522 and NJ548 were identified by 16S rDNA sequence analysis and result showed that all of these four strains belonged to γ-Proteobacteria in Bacteria.The strain NJ062 was determined as genus Pseudoalteromonas sp.,the strain NJ379 belonged to genus Shewanella sp.,and the strain NJ522 and NJ548 were classified as Marinomonas sp..