极地研究

极地研究 ›› 2007, Vol. 19 ›› Issue (2): 131-138.

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南极生境真菌Gliocladium catenulatum T31菌株杀虫活性的研究

牛德庆;田黎;周俊英;郑立;陈靠山;   

  • 出版日期:2007-06-30 发布日期:2007-06-30

A STUDY ON THE INSECTICIDAL ACTIVITY OF ANTARCTIC EPIPHYTE GLIOCLADIUM CATENULATUM T31

  • Online:2007-06-30 Published:2007-06-30

PDF (PC)

1495

被引次数

40

摘要：

本文对影响南极生境真菌Gliocladium catenulatum T31生长和杀虫活性的初始pH值、发酵时间、培养温度等影响因子进行了研究。并以小菜蛾为试虫,以校正死亡率为评价指标,利用微型平板法对真菌T31的杀虫活性进行测试,发酵液杀虫活性最高为60.42%。发酵物乙酸乙酯提取后,丙酮溶解0.33mg/mL浓度下,发酵液提取物杀虫活性最高为82.05%,菌体提取物杀虫活性为76.92%。盆栽试验证明该菌株发酵液对小菜蛾第7d的虫口减退率为54.2%。

关键词: 南极真菌;杀虫活性;小菜蛾;盆栽试验

Abstract:

In this study,the effects of major environmental factors on the growth and insecticidal activity of antarctic fungi Gliocladium catenulatum T31 were investigated.The method of micro-plate was employed.The bioactivity of the strain T31 to Diamondback Moth(Plutella xylostella) was studied with insect mortality as evaluated index.The most insecticidal activity of T31 fermentation broth was 60.42%.After acetic ether extracted T31 fermentation broth repeatedly,dissolved by acetone at the concentration of 0.33mg/mL,the best insecticidal activity of T31 fermentation broth extracts was 82.05% and insecticidal activity of T31 mycelium extracts was 76.92%.The potted experimentation showed that the controlling effect of T31 fermentation broth to Diamondback Moth was 54.2% on the seventh day.