极地研究

极地研究 ›› 2005, Vol. 17 ›› Issue (2): 127-133.

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一株产海藻糖合成酶南极海洋低温细菌的鉴定

俞勇;李会荣;陈波;曾胤新;   

  • 出版日期:2005-06-30 发布日期:2005-06-30

IDENTIFICATION FOR TREHALOSE SYNTHASE- PRODUCING BACTERIAL STRAIN BSW10041 ISOLATED FROM ANTARCTIC SEA WATER

  • Online:2005-06-30 Published:2005-06-30

PDF (PC)

2242

被引次数

14

摘要：

从南极普里兹湾海域海水中分离到一株产海藻糖合成酶的海洋低温细菌BSw10041,革兰氏阴性,杆状,有极生鞭毛,能运动,菌落半透明。进行了常规生理生化和BIOLOG GN2细菌鉴定系统测试,结果表明菌株BSw10041与恶臭假单胞菌(Pseudomonas putida)的表型特征非常相似。为了进一步确定菌株BSw10041的分类学地位,测定了其16S rDNA序列,分析了相关细菌相应序列的同源性,构建了系统发育树,结果表明BSw10041与P．putida的亲缘关系最近。综合上述结果,菌株BSw10041可鉴定为Pseudomonas putida,定名为Pseudomonas putida BSw10041。

关键词: 海藻糖合成酶;低温微生物;Pseudomonas putida;南极

Abstract:

A trehalose synthase-producing bacterial strain BSwl0041 isolated from Antarctic seawater was reported in the present paper. Morphology observation showed it to be a motile gram-negative bacterium with rod shape, polar flagella. The traditional physiological and biochemical methods, together with BIOLOG GN2 system were applied in the bacterial classification. Results showed that strain BSw10041 was similar to Pseudomonas putida in most of the pheno-types. According to the BIOLOG GN2 results, the BSwl0041 is 84. 8% similarity to Pseudomonas putida. To investigate the phylogenetic position of this bacterial strain, a 1658bp of 16S rRNA gene of strain BSw10041 was sequenced and compared with that of related strains. Phylogenetic tree was constructed based on the genetic distance analysis, which revealed that strain BSwl0041 is highest similar to P. putida ( GenBank accession number Z76667). The similarity value between BSwl0041 and P. putida ( Z76667 ) is 99. 9%. Based on the results obtained , strain BSw 10041 was identified as Pseudomonas putida and named for Pseudomonas putida BSw10041.