极地研究 ›› 2009, Vol. 21 ›› Issue (2): 100-108.

• 研究论文 • 上一篇    下一篇

南极阿德雷岛淡水湖沉积物细菌群落研究

王鹏, 王风平   

  1. 同济大学海洋地质国家重点实验室; 国家海洋局第三海洋研究所生物工程重点实验室;
  • 出版日期:2009-06-30 发布日期:2009-06-30

PHYLOGENETIC DIVERSITY OF CULTURABLE BACTERIA IN LAKE SEDIMENT OF ARDLEY ISLAND,ANTARCTIC

WANG Feng, WANG Feng-Beng   

  1. 1 State Key Laboratory of Marine Geology; Tongji University; Shanghai 200092; China; 2 Key Laboratory of Marine Biogenetic Resources; State Oceanic Administration; Xiamen 361005; China
  • Online:2009-06-30 Published:2009-06-30
  • Supported by:

    国家自然科学基金(40606032);; 国家“十一五”大洋专项基金(DYXM-115-02-2-17);; 国家重点基础研究发展计划(973计划)(2007CB815904);; 同济大学青年优秀人才培养行动计划(2006KJ056)资助

摘要:

应用样品直接稀释涂布平板法,从南极阿德雷岛淡水湖沉积物中分离得到34株菌,并进行了生理生化性质检测。根据其16SrDNA全长序列所进行的系统发育分析表明,分离菌株分属于革兰氏阳性菌:节杆菌(Arthrobacter)、红球菌(Rhodococcus)、葡萄球菌(Staphylococcus);gamma-变形细菌(gamma-proteobacteria):假单胞菌(Pseudomonas)、不动杆菌(Acinetobacter)、寡养单胞菌(Stenotrophomonas);beta-变形细菌(beta-proteobacteria):紫色杆菌(Janthinobacterium);拟杆菌门(Cytophaga-Flexibacteria-Bacteroides):假结核棒杆菌(Cytophaga)、土地杆菌(Pedobacter)等9个属。分离的菌株在4℃条件下能产生多种大分子物质水解酶类,其中32%的菌株能产生脂肪酶,18%的菌能够水解明胶或者产生卵磷脂酶。同时对沉积物垂直方向的可培养细菌生物量进行了分析,表明阿德雷岛企鹅排泄物输入量对微生物分布有较大影响。

关键词: 南极, 细菌, 低温水解酶, 企鹅, 南极, 细菌, 低温水解酶, 企鹅

Abstract:

The phylogenetic diversity of culturable bacteria in lake sediment of the Ardley Island,Antarctic was investigated. A total of 34 strains were isolated and 16S rDNA nearly full-length sequence analysis revealed that strains fall in 4 divisions:gram positive bacteria (Arthrobacter,Rhodococcus,Staphylococcus);gamma-proteobacteria (Pseudomonas,Acinetobacter,Stenotrophomonas);beta-proteobacteria (Janthinobacterium);Cytophaga-Flexibacteria-Bacteroides(Cytophaga,Pedobacter). The majority of the bacterial strains ...

Key words: Antarctic, bacteria, cold-adaptive hydrolytic enzymes, penguin