极地研究

极地研究

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Detection of integron integrase genes on King George Island, Antarctica

Verónica Antelo1*, Héctor Romero2 & Silvia Batista1   

  1. 1 Unidad de Microbiología Molecular, Biogem, Instituto de Investigaciones Biológicas Clemente Estable (MEC), Montevideo, C.P. 11600, Uruguay;
    2 Departamento de Ecología y Evolución, Facultad de Ciencias, UDeLaR, Montevideo, C.P. 11400, Uruguay
  • 出版日期:1965-03-30 发布日期:1965-03-30
  • 通讯作者: Verónica Antelo

Detection of integron integrase genes on King George Island, Antarctica

Verónica Antelo1*, Héctor Romero2 & Silvia Batista1   

  1. 1 Unidad de Microbiología Molecular, Biogem, Instituto de Investigaciones Biológicas Clemente Estable (MEC), Montevideo, C.P. 11600, Uruguay;
    2 Departamento de Ecología y Evolución, Facultad de Ciencias, UDeLaR, Montevideo, C.P. 11400, Uruguay
  • Online:1965-03-30 Published:1965-03-30
  • Contact: Verónica Antelo

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摘要： The presence and diversity of class 1 integrase gene (intI) sequences were evaluated by PCR using previously designed primers. Two clone libraries were constructed from DNA in sediment and microbial mat samples collected on Fildes Peninsula, King George Island, Antarctica.The libraries constructed from samples collected at Halfthree Point (HP) and Norma Cove (NC) contained 62 and 36 partial intI sequences, respectively. These sequences clustered into 10 different groups with < 95% amino acid identity. Alignment of the deduced amino acid sequences with those from recognized integron-encoded integrases demonstrated the presence of highly conserved motifs characteristic of intI integrases. The HP library contained 42 nucleotide sequences identical to the class 1 intI gene found in a collection of trimethoprim-resistant (Tmpr) Antarctic Enterobacter sp. isolates, previously collected in the same area. These integrons, located on plasmids, had a genetic organization similar to that of pKOX105 from Klebsiella oxytoca. The 20 remaining HP and NC library sequences were similar to integrase sequences previously determined in a metagenomic analysis of environmental samples. We have demonstrated the presence of integron integrase genes in Antarctic sediment samples. About half these genes were very similar to the class 1 integrons found in humanassociated microbiota, suggesting that they originated from human-dominated ecosystems. The remaining integrase genes were probably associated with endemic bacteria.

关键词: integrase, amplicon library, maritime Antarctica

Abstract: The presence and diversity of class 1 integrase gene (intI) sequences were evaluated by PCR using previously designed primers. Two clone libraries were constructed from DNA in sediment and microbial mat samples collected on Fildes Peninsula, King George Island, Antarctica.The libraries constructed from samples collected at Halfthree Point (HP) and Norma Cove (NC) contained 62 and 36 partial intI sequences, respectively. These sequences clustered into 10 different groups with < 95% amino acid identity. Alignment of the deduced amino acid sequences with those from recognized integron-encoded integrases demonstrated the presence of highly conserved motifs characteristic of intI integrases. The HP library contained 42 nucleotide sequences identical to the class 1 intI gene found in a collection of trimethoprim-resistant (Tmpr) Antarctic Enterobacter sp. isolates, previously collected in the same area. These integrons, located on plasmids, had a genetic organization similar to that of pKOX105 from Klebsiella oxytoca. The 20 remaining HP and NC library sequences were similar to integrase sequences previously determined in a metagenomic analysis of environmental samples. We have demonstrated the presence of integron integrase genes in Antarctic sediment samples. About half these genes were very similar to the class 1 integrons found in humanassociated microbiota, suggesting that they originated from human-dominated ecosystems. The remaining integrase genes were probably associated with endemic bacteria.

Key words: integrase, amplicon library, maritime Antarctica