极地研究

极地研究 ›› 2010, Vol. 22 ›› Issue (2-Chinese): 164-173.DOI: 10.3724/SP.J.1084.2010.00164

• 研究论文 • 上一篇    下一篇

UV-B短期照射对北极冰川小球藻Chlorella sp.的生物学效应

彭小伟1,何剑锋2,罗玮1,李运广1,蔡明红2   

  1. 1.
    2. 中国极地研究中心
  • 收稿日期:2010-02-24 修回日期:2010-04-12 出版日期:2010-06-30 发布日期:2010-06-30
  • 通讯作者: 何剑锋

The physiological and biochemical responses of an Arctic microalgae (Chlorella sp.) to UV-B radiation

  • Received:2010-02-24 Revised:2010-04-12 Online:2010-06-30 Published:2010-06-30

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被引次数

17

摘要：

以一种分离自北极冰川融水的淡水微藻（Chlorella sp.）为实验材料，设置两个不同强度UV-B（45μw/cm2、76μw/cm2）照射处理、每4小时取样检测，对其生化组分进行分析，了解微藻对UV-B辐射的响应。研究结果显示小球藻经辐射处理后，其生化成分发生变化：（1）在较低强度的UV-B（45μw/cm2）辐射下，叶绿素a（Chla）和类胡萝卜素（Car）含量先升后降，且在4h时有最大值；高强度UV-B（76μw/cm2）辐射下，Chla和Car含量随辐射时间延长基本呈下降趋势。（2）超氧阴离子（O2?ˉ）和丙二醛（MDA）含量基本随辐照时间的延长和辐照强度的增大提高，过氧化氢（H2O2）含量变化虽随辐照时间的延长呈上升趋势，但直到12h是才表现为明显的提高。（3）超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性在低UV-B处理下，随辐照时间的延长呈升高趋势；在高UV-B辐射下，SOD活性先升后降，CAT活性上下波动。(4)UV-B辐射使小球藻脂肪酸组成发生了变化。这些结果表明：UV-B，尤其是高强度或较高剂量的UV-B会对北极冰川微藻带来不利的影响。

关键词: UV-B辐射 小球藻 生化组分

Abstract:

The response of an Arctic microalgae strain (Chlorella sp.) to the enhancement of UV-B radiation was explored in this paper. The effects of three different intensity (0 μw/cm2, 45 μw/cm2, 76 μw/cm2 ) within 12 h radiation following with 4 h non-radiation recovery were studied, while the biochemical components were tested by sampling every 4 h. Our results showed that: (1) Under the lower intensity of UV-B (45μw/cm2) radiation, Chla and Car content increased in the beginning 4 h and then decreased. The maximum value presented after 4 hrs. Meanwhile, under high-intensity UV-B (76μw/cm2) radiation, Chla and CAR content constantly decreased. (2) The content of O2?ˉ and MDA enhanced along with radiation time and intensity. Moreover, H2O2 content also increased with irradiation time; significant vary happened after 8 h radiation. (3) SOD, CAT activity under the lower UV-B treatment, increased with irradiation time as well. Under higher UV-B radiation, SOD activity increased at the beginning 4 hrs and then decreased constantly, CAT activity fluctuated from time to time.（4）The fatty acid composition of Chlorella was influenced. The results supported that UV-B had adverse effects on the Arctic glaciers microalgae, and the effect of hurt was more significant under high-intensity or high doses of UV-B.

Key words: UV-B radiation, Chlorella sp., biochemical composition