极地研究

极地研究 ›› 2006, Vol. 18 ›› Issue (2): 130-135.

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南极菌Pseudoalteromonas sp．S-15-13产胞外多糖的研究及其分子鉴定

李江;陈靠山;林学政;李光友;   

  • 出版日期:2006-06-30 发布日期:2006-06-30

PRODUCTION OF EXTRACELLULAR POLYSACCHARIDE AND PHYLOGENETIC ANALYSIS OF ANTARCTIC BACTERIAL PSEUDOALTEROMONAS SP.S-15-13

  • Online:2006-06-30 Published:2006-06-30

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摘要：

本文对影响南极细菌S-15-13生长和胞外多糖产量的主要环境因子进行了研究,同时采用16S rDNA序列分析及系统发育分析方法对其进行了分子鉴定。结果表明：菌株S-15-13最佳产糖条件为：培养时间,56h；培养温度,8℃；碳源,1．0%葡萄糖；NaCl浓度,3．0%；pH,6．0—7．0。16S rDNA序列分析和系统发育分析表明,菌株S-15-13属于假交替单胞菌属(Pseudoaltero- monas)。

关键词: 南极细菌;胞外多糖;环境因子;系统发育分析

Abstract:

In this study,the effects of major environmental factors on the growth and EPS production of Antarctic bacteria S-15-13 were investigated.Meanwhile,the cloned 16S rDNA and other 24 related sequences were included in the consensus phylogenetic analysis by using neighboring- joining method.The results showed that the optimal conditions for EPS production were culture period,56h;growth temperature,8℃;carbon source,1.0% glucose;NaCl concentration,3. 0%;pH 6.0-7.0.Phylogenetic analysis indicated that strain S-15-13 belong to Pseudoaltero- monas sp.