极地研究

极地研究 ›› 2006, Vol. 17 ›› Issue (2-English): 81-89.

• 目次 •    下一篇

Phylogenetic analysis of bacteria in sea ice brine sampled from the Canada Basin, Arctic Ocean

 Li Huirong1, Yu Yong1, Chen Bo1, Zeng Yinxin1 and Ren Daming2   

  1. 1 SOA Key Laboratory of Polar Science, Polar Research Institute of China, Shanghai 200136, China;
    2 State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China
  • 出版日期:1956-06-30 发布日期:1956-06-30
  • 通讯作者: Li Huirong

Phylogenetic analysis of bacteria in sea ice brine sampled from the Canada Basin,Arctic Ocean

Li Huirong1, Yu Yong1, Chen Bo1, Zeng Yinxin1 and Ren Daming2   

  1. 1 SOA Key Laboratory of Polar Science, Polar Research Institute of China, Shanghai 200136, China;
    2 State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China
  • Online:1956-06-30 Published:1956-06-30
  • Contact: Li Huirong

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摘要：

Bacterial diversity in sea ice brine samples which collected from four stations located at the Canada Basin,Arctic Ocean was analyzed by PCR-DGGE.T wenty-three 16S rDNA sequences of bacteria obtained from DGGE bands were cloned and sequenced.Phylogenetic analysis clustered these sequences within γ-prote obacteria, Cytophaga-Flexibacter-Bacteroides (CFB) group, Firmicutes and A ctinobacteria. The phylotype of Pseudoalteromonas in the γ-proteobacteria was predominant and members of the CFB group and γ-proteobacteria were highly abundant in studied sea ice brine samples.

关键词: sea ice brine, Arctic, bacteria, 16S rDNA, phylogenetic

Abstract:

Bacterial diversity in sea ice brine samples which collected from four stations located at the Canada Basin,Arctic Ocean was analyzed by PCR-DGGE.T wenty-three 16S rDNA sequences of bacteria obtained from DGGE bands were cloned and sequenced.Phylogenetic analysis clustered these sequences within γ-prote obacteria, Cytophaga-Flexibacter-Bacteroides (CFB) group, Firmicutes and A ctinobacteria. The phylotype of Pseudoalteromonas in the γ-proteobacteria was predominant and members of the CFB group and γ-proteobacteria were highly abundant in studied sea ice brine samples.

Key words: sea ice brine, Arctic, bacteria, 16S rDNA, phylogenetic