极地研究

极地研究 ›› 2017, Vol. 29 ›› Issue (2): 228-235.DOI: 10.13679/j.jdyj.2017.2.228

• 研究论文 • 上一篇    下一篇

两株北极褐藻DNA条形码序列的记录

刘晨临1,2  林学政1   

  1. 1. 国家海洋局第一海洋研究所, 山东 青岛 266061
    2. 青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室, 山东 青岛 266000
  • 收稿日期:2016-01-11 修回日期:2016-04-08 出版日期:2017-06-30 发布日期:2017-06-30
  • 通讯作者: 刘晨临
  • 基金资助:

    南北极环境综合考察与评估专项(CHINARE2012-03-05，CHINARE2016-03-05)资助

The DNA barcoding records of two strains of brown seaweeds from Arctic

Liu Chenlin1,2, Lin Xuezheng1     

  • Received:2016-01-11 Revised:2016-04-08 Online:2017-06-30 Published:2017-06-30

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摘要：

2012年第5次北极科学考察期间, 分别在白令海海域和冰岛附近海域各采集到1株褐藻。描述了其形态学特征, 并利用多种DNA条形码序列对其进行了分子鉴定。形态学和基于ITS2序列构建的系统进化树均表明白令海北部海域采集到的AE5-8为孔叶藻亚种 Agarum clathratum subsp. Clathratum, 这一亚种起源于日本北海道, 经过阿拉斯加到北美的东西海岸都有分布。形态学特征及ITS序列和cox1序列均表明在冰岛附近海域采集到的AE5-12是瘤状囊叶藻(Ascophyllum nodosum), 瘤状囊叶藻在北大西洋沿岸属于常见种, 但是北太平洋海岸未见其分布的报道。

关键词: DNA条形码, 北极褐藻, 孔叶藻, 瘤状囊叶藻

Abstract:

During the fifth Chinese National Arctic Research Expedition in 2012, two strains of brown seaweeds were collected from the Bering Sea and Greenland Sea, respectively. Their morphological characteristics have been described, and taxonomic status has been studied based on DNA barcoding sequences. Both morphological characteristics, and phylogenetic relationships based on ITS2 sequences, proved that strain AE5-8 from the Bering Sea belonged to Agarum clathratum subsp. clathratum. This species can be found from Hokkaido, Japan, via Alaska to the west and east coasts of North America. The Greenland Sea alga AE5-12 belonged to Ascophyllum nodosum based on the phylogenetic trees of DNA barcoding sequences ITS and COX1. A. nodosum is a dominant fucoid seaweed occurring throughout the North Atlantic. No North Pacific representatives of this species have been reported.

Key words: DNA barcoding, Arctic brown seaweed, Agarum clathratum, Ascophyllum nodosum