极地研究

极地研究 ›› 2016, Vol. 28 ›› Issue (3): 424-429.DOI: 10.13679/j.jdyj.2016.3.424

• 研究简报 • 上一篇    

南极青霉菌Penicillium sp. S-1-16摇瓶发酵条件的初步优化

叶科元1,2，唐潮1,2，卢小玲2，吴文惠1，刘小宇2   

  1. 1. 上海海洋大学食品学院，上海 201306
    2. 第二军医大学海洋生物医药研究中心，上海 200433
  • 收稿日期:2015-02-06 修回日期:2015-11-02 出版日期:2016-09-30 发布日期:2016-09-30
  • 通讯作者: 吴文惠
  • 基金资助:

    国家863计划(2012AA092105)、国家自然科学基金项目(41306197)、广东省海洋局公益项目(GD2012-D01-001)资助

Pre-optimization of Fermentation Conditions for the Antarctica Fungus Penicillium sp. S-1-16

Ye Keyuan1,2，Tang Chao1,2，Lu Xiaoling2，Wu Wenhui1，Liu Xiaoyu2   

  • Received:2015-02-06 Revised:2015-11-02 Online:2016-09-30 Published:2016-09-30

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摘要： 对一株具有细胞毒活性的南极青霉属真菌Penicillium sp. S-1-16进行发酵条件的初步优化。通过对基础发酵培养基、发酵温度及培养基配制用水的筛选，以次级代谢产物量为主要指标，同时参考生物量及细胞毒活性指标来进行初步优化，获得一个较优的摇瓶发酵条件：酵母提取粉0.3%，蛋白胨0.5%，麦芽糖0.3%，葡萄糖1%，蔗糖5%，自来水，初始pH=7.0，接种量15%，18°C，180 r·min-1发酵15 d。采用初步优化后的条件进行发酵，与初始发酵条件相比，其次级代谢产物量提高到568.75%，对A549肿瘤细胞的抑制作用增强至265%，并且对HeLa肿瘤细胞和SGC-7901肿瘤细胞的抑制率分别从0提高到48.19%和69.18%，效果显著，为获得菌株中活性次级代谢产物奠定了基础。

关键词: 南极真菌, Penicillium sp. S-1-16, 细胞毒活性, 发酵初步优化

Abstract: Culture media and fermentation conditions for cultivation of Penicillium sp. S-1-16 isolated from an Antarctic sea cucumber sample were investigated to improve the yield of secondary metabolites with modest cytotoxic activity against three cancer cell lines. The optimum fermentation conditions, including medium, temperature, and type of water, were identified based on fungal biomass, secondary metabolite production, and antitumor activity. The initial fermentation conditions were as follows: 0.3% yeast powder, 0.5% peptone, 0.3% maltose, 1% glucose, 5% sucrose, tap water, a 15% inoculum size, an initial pH of 7.0, and incubation at 18°C, with shaking at 180 r·m-1, for 15 d. Under the optimized fermentation conditions , the amount of secondary metabolites and their cytotoxic activity increased by 568.75% and 265%, respectively, compared with the initial conditions. Additionally, the secondary metabolites’ inhibition of the growth of HeLa and SGC-7901 cells increased from 0 to 48.19% and 69.18%, respectively, after the optimization. These results provide clues for subsequent separation and purification of secondary metabolites derived from the Penicillium sp. S-1-16 strain.

Key words: Antarctica fungus, Penicillium sp. S-1-16, Cytotoxic activity, Pre-optimization