极地研究

极地研究 ›› 2016, Vol. 28 ›› Issue (3): 331-335.DOI: 10.13679/j.jdyj.2016.3.33

• 研究论文 • 上一篇    下一篇

北极海洋放线菌Streptomyces sp. 604F中的抗霉素A和伏尔加霉素类成分

江昇平1,2，田晓清1，廖丽3，杨桥1，陆亚男1，马丽艳1，陈波3，樊成奇1   

  1. 1. 农业部东海与远洋渔业资源开发利用重点实验室，中国水产科学研究院东海水产研究所，上海 200090
    2. 上海海洋大学，上海 201306
    3. 中国极地研究中心，上海 200136
  • 收稿日期:2015-02-12 修回日期:2015-04-21 出版日期:2016-09-30 发布日期:2016-09-30
  • 通讯作者: 樊成奇
  • 基金资助:

    国家自然科学青年基金(41406181)、国家高技术研究发展计划项目(2012AA092105)、国家海洋局“南北极环境综合考察与评估”专项(CHINARE 2015-04-03)和上海市自然科学基金(13ZR1462700)资助

Antimycin A and Vulgamycin Derivatives from the Arctic Marine Actinomycete Streptomyces sp. 604F

Jiang Shengping1,2，Tian Xiaoqing1，Liao Li3，Yang Qiao1，Lu Yanan1，Ma Liyan1，Chen Bo3，Fan Chengqi1   

  • Received:2015-02-12 Revised:2015-04-21 Online:2016-09-30 Published:2016-09-30

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摘要： 极地微生物代谢产生芳香族硝基化合物、肽类、萜类等多种骨架类型的化学成分。为从北极放线菌中发现结构新颖、活性显著的小分子化合物，本研究采用Sephadex LH-20凝胶和半制备HPLC等柱层析分离纯化化合物手段，结合LC-MS、1H-和13C-NMR等波谱学数据和文献对比进行结构解析，从链霉菌Streptomyces sp. 604F提取物中分离鉴定出抗霉素A1b(1)、抗霉素A4a(2)、抗霉素A2a(3)、抗霉素A3a(4)、抗霉素A1a(5)以及伏尔加霉素(6)和8-脱氧伏尔加霉素(7)这7个化合物。化合物1—7是首次从北极海洋沉积物来源的微生物中发现，而且是首次从同一菌株中发现抗霉素A和伏尔加霉素这两类化合物。

关键词: 北极海洋放线菌, 链霉菌604F, 抗霉素A, 伏尔加霉素

Abstract: Various types of constituents, including aromatic nitro compounds, peptides, and terpenoids, have been reported from different polar microorganisms. In our investigation of secondary metabolites of Arctic actinomycete with new structures or potent bioactivity, five antimycin A derivatives, Antimycin A1b (1), Antimycin A4a (2), Antimycin A2a (3), Antimycin A3a (4), Antimycin A1a (5), together with Vulgamycin (6) and its derivative 8-deoxyenterocin (7), were obtained from Streptomyces sp. 604F. The compounds were purified by Sephadex LH-20 column chromatography and semi-preparative high-performance liquid chromatography, and their structures were determined by comparing their liquid chromatograph-mass spectrometry and 1H- and 13C-NMR data with those of previously reported compounds. This is the first report of compounds 1—7 from microbes derived from Arctic sediment, and it is the first time that antimycin and vulgamycin compounds were identified as metabolites from the same source.

Key words: Arctic actinomycete, Streptomyces sp. 604F, Antimycina, Vulgamycin