极地研究

极地研究 ›› 2015, Vol. 27 ›› Issue (3): 319-325.DOI: 10.13679/j.jdyj.2015.3.319

• 研究论文 • 上一篇    下一篇

两株极地真菌次级代谢产物的研究

闫凝星 方莎莎 刘靖堂 卢小玲 刘小宇 焦炳华   

  1. 第二军医大学生物化学与分子生物学教研室，上海 200433
  • 收稿日期:2014-12-17 修回日期:2015-03-10 出版日期:2015-09-30 发布日期:2015-09-30
  • 通讯作者: 闫凝星
  • 基金资助:

    海洋天然产物化合物库;三株北极真菌抗肿瘤活性次级代谢产物及其作用机制研究;极地药源微生物代谢产物化学多样性及药物候选化合物研究

RESEARCHES OF THE SECONDARY METABOLITES OF TWO ARCTIC-DERIVED FUNGUS

Yan Ningxing, Fang Shasha, Liu Jingtang, Lu Xiaoling, Liu Xiaoyu, Jiao Binghua   

  1. Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
  • Received:2014-12-17 Revised:2015-03-10 Online:2015-09-30 Published:2015-09-30

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摘要： 采用硅胶柱层析、凝胶柱层析和HPLC等色谱技术，对极地真菌Articulospora sp. Z1-1和Penicillium sp. S-1-10的发酵液提取物进行分离纯化，共得到9个化合物，通过理化性质及波谱学方法并比较相关文献，确定化合物结构为3, 7-二羟基-9-甲氧基-1-甲基-苯并[c]色烯-6-酮(1), 1, 4-二羟基甲苯(2),间羟基苄醇(3), 对羟基苯乙醇(4),邻苯二甲酸二丁酯(5),1, 7-二羟基-3-羟甲基-8-甲基-二苯并[b, e]氧杂卓-13, 14-二酮(6),邻苯二甲酸二(2-乙基己)酯(7),4-十三烷基-苯甲醛（8）和(Z)-7-十四烯酸（9）。其中,化合物1—6首次从Articulospora sp.属真菌中获得,化合物8为新天然产物。化合物1和4被证明具有抑制稻瘟霉的作用,化合物2有较好的抗菌和细胞毒活性,化合物3有较强的抗氧化活性。

关键词: 极地真菌, 节枝孢属, 青霉属次级代谢产物, 生物学活性

Abstract: Nine compounds were obtained from the fermentation broth of the Arctic-derived fungus Articulospora sp. Z1-1 and Penicillium sp. S-1-10. The broth extract was isolated and purified by column chromatography on silica -gel, Sephadex LH-20 and HPLC. The structures were elucidated by physical and chemical properties and spectral analysis, and comparison with the data of literatures. The nine compounds were identified as 3,7-dihydroxy-9 -methoxy-1-methyl-benzo[c]chromen-6-one (1), 1,4-dihydroxytoluene (2), 3-hydroylmethy phenol(3), 2-hydroxyphenethyl alcohol (4), dibutyl phthalate (5), 1,7-dihydroxy-3-hydroxyl-8methyl-dibenzo[b, e] oxepine-13, 14-dion -e (6), bis (2-ethylhexyl) phthalate (7), 4-tridecyl-benzaldehyde (8) and (Z)-7-tetradecenoic acid (9). Compounds 1–6 were firstly isolated from Articulospora sp. Z1-1 and compound 8 was new natural product. Compound 1 and 4 exhibited antifungal activity against conidia of Pyricularia oryzae, and compound 2 was shown to have antifungal and cytotoxic activity, and compound 3 exhibited strong antioxidant activity.

Key words: Arctic-derived fungus, Articulospora sp.Z1-1, Penicillium sp.S-1-10, secondary metabolites, biological activities