极地研究

极地研究 ›› 2010, Vol. 21 ›› Issue (2-English): 193-203.DOI: 10.3724/SP.J.1085.2010.00193

• 研究论文 • 上一篇    下一篇

The adaptability of three Arctic microalgae to different low temperatures

Xia Lihua(夏利花)1,2, He Jianfeng(何剑锋)1*, Zhang Fang(张芳)1, Gao Yan(高岩)3, Zhang Rumin(张汝民)3 and Cui Shikai(崔世开)4,1
  

  1. 1 Key Laboratory for Polar Science of the State Oceanic Administration, Polar Research Institute of China, Shanghai 200136, China;
    2 College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010018, China;
    3 School of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Lin’an 311300, China;
    4 Shanghai Ocean University, Shanghai 201306, China
     
  • 收稿日期:2010-08-03 修回日期:2010-11-22 出版日期:1960-06-30 发布日期:1960-06-30
  • 通讯作者: 何剑锋
  • 基金资助:

    国家自然科学基金;上海市科委国际合作项目;国家海洋局青年海洋科学基金;国家海洋局海洋环境科学和数值模拟重点实验室开放基金;中国极地研究中心极地科学青年创新基金;中国极地科学战略研究基金

The adaptability of three Arctic microalgae to different low temperatures

Xia Lihua(夏利花)1,2, He Jianfeng(何剑锋)1*, Zhang Fang(张芳)1, Gao Yan(高岩)3, Zhang Rumin(张汝民)3 and Cui Shikai(崔世开)4,1
  

  1. 1 Key Laboratory for Polar Science of the State Oceanic Administration, Polar Research Institute of China, Shanghai 200136, China;
    2 College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010018, China;
    3 School of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Lin’an 311300, China;
    4 Shanghai Ocean University, Shanghai 201306, China
  • Received:2010-08-03 Revised:2010-11-22 Online:1960-06-30 Published:1960-06-30
  • Contact: He Jianfeng

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摘要：

In order to study the adaptability of Arctic microalgae to different environmental temperatures, the growth curves and antioxidase system of three microalgae (Skeletonema marinoi, Chlorella sp. and Chlamydomonas sp.) that were separated from the Ny-Ålesund, the high Arctic, at different low temperatures (0℃, 4℃ and 8℃) were determined. The result showed that the adaptability of the microalgae to temperatures depended on the species. The growth rate, SOD and CAT activities of Skeletonema marinoi were the highest at 4℃, but MDA content was the lowest. The growth rate and enzyme activity of Chlorella sp. were the highest at 8℃, while the lowest MDA content presented at 0℃. The growth of Chlamydomonas sp. at the different temperatures was not so significant, the lowest MDA content presented at 8℃. The change of antioxidase system also depended on species and temperatures. Three indexes of antioxidase system of Skeletonema marinoi between 0℃ and 4℃ showed extremely significant difference (p<0.01). SOD activity of Skeletonema marinoi and Chlorella sp.between 0℃ and 8℃ showed significant difference (p<0.05), and the other two indexes of them differed insignificantly. Antioxidase systems of Chlamydomonas sp. at the three temperatures differed insignificantly. In conclusion, the three microalgae had good adaptability to the three temperatures; their MDA content presented a low level, and had unique physiological mechanism to adapt to the environment with different low temperatures.

关键词: Arctic microalga, adaptability, MDA, SOD, CAT

Abstract:

In order to study the adaptability of Arctic microalgae to different environmental temperatures, the growth curves and antioxidase system of three microalgae (Skeletonema marinoi, Chlorella sp. and Chlamydomonas sp.) that were separated from the Ny-Ålesund, the high Arctic, at different low temperatures (0℃, 4℃ and 8℃) were determined. The result showed that the adaptability of the microalgae to temperatures depended on the species. The growth rate, SOD and CAT activities of Skeletonema marinoi were the highest at 4℃, but MDA content was the lowest. The growth rate and enzyme activity of Chlorella sp. were the highest at 8℃, while the lowest MDA content presented at 0℃. The growth of Chlamydomonas sp. at the different temperatures was not so significant, the lowest MDA content presented at 8℃. The change of antioxidase system also depended on species and temperatures. Three indexes of antioxidase system of Skeletonema marinoi between 0℃ and 4℃ showed extremely significant difference (p<0.01). SOD activity of Skeletonema marinoi and Chlorella sp.between 0℃ and 8℃ showed significant difference (p<0.05), and the other two indexes of them differed insignificantly. Antioxidase systems of Chlamydomonas sp. at the three temperatures differed insignificantly. In conclusion, the three microalgae had good adaptability to the three temperatures; their MDA content presented a low level, and had unique physiological mechanism to adapt to the environment with different low temperatures.

Key words: Arctic microalga, adaptability, MDA, SOD, CAT