极地研究

极地研究 ›› 2008, Vol. 19 ›› Issue (1-English): 77-84.

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Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice

 Li Xiaohui1,2, Yu Yong3, Li Huirong3, Zhang Lin2, Jiang Xinyin1 and Ren Daming1   

  1. 1 State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China;
    2 College of Food Science and Technology, Shanghai Fisheries University, Shanghai 200090, China;
    3 Polar Research Institute of China, Shanghai 200129, China
  • 出版日期:1958-03-30 发布日期:1958-03-30
  • 通讯作者: Li Xiaohui

Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice

 Li Xiaohui1,2, Yu Yong3, Li Huirong3, Zhang Lin2, Jiang Xinyin1 and Ren Daming1   

  1. 1 State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China;
    2 College of Food Science and Technology, Shanghai Fisheries University, Shanghai 200090, China;
    3 Polar Research Institute of China, Shanghai 200129, China
  • Online:1958-03-30 Published:1958-03-30
  • Contact: Li Xiaohui

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摘要：

Arctic sea ice in the polar region provides a cold habitat for microbial community.Arctic sea ice microorganisms are revealed to be of considerable importance in basic research and potential in biotechnological application. This paper investigated the culture condition and extracellular hydrolase of 14 strains of different Arctic sea ice bacteria. The results showed that optimal growth temperature of strains is 15℃or 20℃. The optimal pH is about 8.0. They hardly grow at acid condition. 3% NaCl is necessary for better growth. These strains have different abilities in producing amylase, protcase, cellulase and lipase. Pseudoalteronomas sp. Bsi429 and Pseudoalteronomas sp. Bsi539 produced both cellulose, protease and lipase. These results provide a basis for further developing and exploiting the cold adapted marine enzyme resources.

关键词: Arctic sea ice microorganisms, culture condition, Pseudoalteronomas, hydrolase

Abstract:

Arctic sea ice in the polar region provides a cold habitat for microbial community.Arctic sea ice microorganisms are revealed to be of considerable importance in basic research and potential in biotechnological application. This paper investigated the culture condition and extracellular hydrolase of 14 strains of different Arctic sea ice bacteria. The results showed that optimal growth temperature of strains is 15℃or 20℃. The optimal pH is about 8.0. They hardly grow at acid condition. 3% NaCl is necessary for better growth. These strains have different abilities in producing amylase, protcase, cellulase and lipase. Pseudoalteronomas sp. Bsi429 and Pseudoalteronomas sp. Bsi539 produced both cellulose, protease and lipase. These results provide a basis for further developing and exploiting the cold adapted marine enzyme resources.