极地研究

极地研究 ›› 2002, Vol. 13 ›› Issue (2-English): 157-168.

• 目次 • 上一篇    

Isolation and characteristics of one marine psychrotrophic cellulase-generating bacterium Ar/w/b/75°/10/5 from Chuckchi Sea, Arctic

 Zeng Yinxin and Chen Bo   

  1. Polar Research Institute of China, Shanghai 200129, China
  • 出版日期:1952-06-30 发布日期:1952-06-30
  • 通讯作者: Zeng Yinxin

Isolation and characteristics of one marine psychrotrophic cellu-lase - generating bacterium Ar/w/b/75°/10/5 from Chuckchi Sea, Arctic

 Zeng Yinxin and Chen Bo   

  1. Polar Research Institute of China, Shanghai 200129, China
  • Online:1952-06-30 Published:1952-06-30
  • Contact: Zeng Yinxin

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摘要：

Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the bio-technological applications. A psychrotrophic bacterium (strain Ar/w/b/75°/10/5) , producing cellulose at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chuckchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15℃. No cell growth was observed at 351 or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35℃ and pH8. Cellulase was irreversibly inactivated when incubated at 55℃ within 30 min. Enzyme can be kept stable at the temperature no higher than 25℃. Of special interest was that this bacterium produced various extracellular enzymes including cellulase, amylase, agar hydrolase and protease, at low or moderate temperature conditions, which is certainly of it potential value for applications.

关键词: psychrotrophic, bacterium, cellulase, Chuckchi Sea, Arctic

Abstract:

Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the bio-technological applications. A psychrotrophic bacterium (strain Ar/w/b/75°/10/5) , producing cellulose at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chuckchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15℃. No cell growth was observed at 351 or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35℃ and pH8. Cellulase was irreversibly inactivated when incubated at 55℃ within 30 min. Enzyme can be kept stable at the temperature no higher than 25℃. Of special interest was that this bacterium produced various extracellular enzymes including cellulase, amylase, agar hydrolase and protease, at low or moderate temperature conditions, which is certainly of it potential value for applications.

Key words: psychrotrophic, bacterium, cellulase, Chuckchi Sea, Arctic