极地研究

极地研究 ›› 2025, Vol. 37 ›› Issue (3): 541-550.DOI: 10.13679/j.jdyj.20240053

• 研究论文 • 上一篇    下一篇

高通量微液滴技术在南大洋沉积物微生物分离培养中的应用研究

刘忆思1,2, 张瑾2, 毛梦婷1, 刘仁宗2, 段泽东2,3, 陈波2, 廖丽2,3   

  1. 1上海海洋大学海洋科学与生态环境学院, 上海 201306; 
    2自然资源部极地科学重点实验室, 中国极地研究中心(中国极地研究所), 上海 200136; 
    3上海交通大学海洋学院, 上海 200240

  • 收稿日期:2024-05-13 修回日期:2024-06-25 出版日期:2025-09-30 发布日期:2025-09-25
  • 通讯作者: 廖丽
  • 基金资助:
    国家重点研发计划

Application and culture efficiency of the high-throughput microdroplet technique in the isolation and cultivation of microorganisms from Southern Ocean sediments

LIU Yisi1,2, ZHANG Jin2, MAO Mengting1, LIU Renzong2, DUAN Zedong2,3, CHEN Bo2, LIAO Li2,3   

  1. 1College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China;
    2Key Laboratory of Polar Science, MNR, Polar Research Institute of China, Shanghai 200136, China;
    3School of Oceanography, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2024-05-13 Revised:2024-06-25 Online:2025-09-30 Published:2025-09-25

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摘要： 为评估微液滴技术在极地微生物分离培养中的可行性, 探索其对提升微生物培养效率的潜力, 本研究采用高通量微液滴包裹技术, 开展南大洋沉积物样品的微生物分离与培养研究。研究结果表明, (1)与传统平板分离法相比, 高通量微液滴法分离培养出的菌株类群存在差异。尽管两种方法都主要分离出了盐单胞菌属(Halomonas), 但它们在菌株占比上有所不同。(2)两种方法都成功分离出了对方未能培养出的其他菌株类群, 这表明结合使用这两种技术可以有效增强对微生物物种多样性的覆盖。(3)在本研究测试的条件下, 相较于传统平板分离法, 高通量微液滴法分离培养的菌株种类总数不占优势, 但在单位时间内能够获得更多的非冗余扩增子序列可变体(amplicon sequence variant), 体现了其在提升微生物分离培养效率上的潜力。以上结论可为探索高通量微液滴包裹技术在极地微生物分离培养中的应用提供科学依据, 也为今后在极地微生物的分离培养中进一步优化此类方法提供经验参考。

关键词: 高通量微液滴技术, 菌株分离培养,  群落结构,  沉积物微生物,  南大洋

Abstract: To evaluate the feasibility of microdroplet technology for the isolation and cultivation of polar microorganisms, and to explore its potential for enhancing microbial cultivation efficiency, this study utilized high-throughput microdroplet encapsulation technology to conduct research on the isolation and cultivation of microorganisms from sediment samples collected in the Southern Ocean. The results show that: (1) Compared with the traditional spread plate method, differences were observed in the bacterial groups isolated and cultivated using the high-throughput microdroplet method. Although both methods primarily isolated Halomonas, the resultant proportions differed. (2) Both methods successfully isolated other strain groups that the other method failed to cultivate. This indicates that the combined use of these two techniques can effectively increase microbial species diversity. (3) Under the conditions of this study, the total number of strains isolated and cultured by the high-throughput microdroplet method was less than that isolated by the traditional spread plate method, but more non-redundant amplicon sequence variants were detected with the high-throughput method than with the traditional spread plate method per unit time, which reflects the potential of the high-throughput method for improving the efficiency of microbial separation. This study provides a scientific basis for exploring the application of high-throughput microdroplet encapsulation technology in the isolation and cultivation of polar microorganisms and offers insight and a reference for further optimization of such methods in the isolation and cultivation of polar microorganisms in future studies.

Key words: high-throughput microdroplet technique,  strain isolation and cultivation,  community structure,  sediment microorganisms,  Southern Ocean