极地研究 ›› 2020, Vol. 32 ›› Issue (1): 68-81.DOI: 10.13679/j.jdyj.20190051

• 研究论文 • 上一篇    下一篇

南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测

王飞1,丛柏林2,张朝晖2,杨黄浩1   

  1. 1.福州大学, 福建 福州 350108;
    2.自然资源部第一海洋研究所, 山东 青岛 266061
  • 收稿日期:2019-09-24 修回日期:2019-11-14 出版日期:2020-03-30 发布日期:2020-03-30
  • 通讯作者: 丛柏林
  • 基金资助:

    “十三五”国家重点研发计划(2018YFC1406701)资助

Isolation of culturable strains from Antarctic Ross Sea sediments and study of extracellular hydrolase activity

Wang Fei1, Cong Bailin2, Zhang Zhaohui2, Yang Huanghao1   

  1. 1.Fuzhou University, Fu Zhou 350108, China;
    2.First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
  • Received:2019-09-24 Revised:2019-11-14 Online:2020-03-30 Published:2020-03-30
  • Contact: Bailin Cong

摘要:

研究了南极罗斯海地区沉积物中可培养微生物多样性, 并检测了菌株胞外酶活性, 为开发利用南极资源奠定基础。采用Zobell 2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌, 分别通过16S rDNA18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌, 细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium); 78株细菌菌株中有2株与模式菌株的16S rDNA基因序列相似性小于97%, 可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明, 获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源, 为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。

关键词: 南极罗斯海, 可培养菌株, 胞外酶

Abstract:

To evaluate the potential of Antarctic microbial resources for exploitation, we isolated culturable bacteria from sediments collected from the Ross Sea region in Antarctica and examined their diversity and extracellular enzyme activities. Pure cultured bacteria and fungi were isolated using Zobell 2216E medium and potato dextrose agar(PDA). Diversity and phylogeny of culturable microorganisms were analyzed using 16S rDNA and 18S/ITS sequencing. We found 79 strains of bacteria and 54 strains of fungi, of which Psychrobacter and Cladosporium were dominant. Phenotypic similarity with model strain was low (less than 97%) in two bacterial strains, indicating the potential discovery of new species. Results from API20NE tests show that most strains have extracellular hydrolase activity. This study provides new information on culturable microbial resources in Antarctica and lays the foundation for exploitation of Antarctic microbial resources.

Key words: Ross Sea, culturable strains, ectoenzyme