极地研究

极地研究 ›› 2016, Vol. 28 ›› Issue (1): 34-41.DOI: 10.13679/j.jdyj.2016.1.034

• 研究论文 • 上一篇    下一篇

南极菲尔德斯半岛土壤可培养细菌多样性分析

杨晓1,2 丁慧1,2 臧家业2 张波涛1 赵倩3 丛柏林2 董龙龙4 岳同辉4 张斌5 王能飞2   

  1. 1青岛大学化学科学与工程学院, 山东 青岛 266071; 2国家海洋局第一海洋研究所 国家海洋局海洋生物活性物质重点实验室, 山东 青岛 266061; 3成都中医药大学药学院, 四川 成都 611137; 4青岛科技大学生物工程与技术系, 山东 青岛 266042; 5山东省农业科学院生物技术研究中心, 山东 济南 250100
  • 收稿日期:2015-03-16 修回日期:2015-06-01 出版日期:2016-03-30 发布日期:2016-03-30
  • 通讯作者: 王能飞
  • 基金资助:

    极地专项(CHINARE-2014-02-01, CHINARE-2014-04-01-07，CHINARE-2014-01-06)及中央级公益性科研院所基本科研业务费专项资金（2015T04）资助

DIVERSITY OF CULTURABLE BACTERIA IN SOIL FROM FILDES PENINSULA, ANTARCTIC

Yang Xiao1,2, Ding Hui1,2, Zang Jiaye2, Zhang Botao1, Zhao Qian3, Cong Bailin2, Dong Longlong4, Yue Tonghui4, Zhang Bin5, Wang Nengfei2   

  1. 1Qingdao University, Qingdao 266071, China； 2Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, Qingdao 266061, China； 3School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China； 4Department of Bioengineering and Biotechnology, Qingdao University of Science and Technology, Qingdao 266042, China； 5Bio-Tech Research Centre, Shandong Academy of Agricultural Sciences, Jinan 250100, China
  • Received:2015-03-16 Revised:2015-06-01 Online:2016-03-30 Published:2016-03-30

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摘要： 对中国第29次南极科学考察采自南极菲尔德斯半岛区域16个站位的土壤样品进行了细菌的分离培养。经细菌16S rDNA序列测定及系统发育分析，共鉴定得到3个门，13个属的67株细菌。其中优势门为放线菌门（Actinobacteria）和变形菌门（Proteobacteria），优势属为节杆菌属（Arthrobacter）和假单胞菌属（Pseudomonas）。研究还对受到不同外界动物因素影响的4个典型站位的土壤理化性质进行了测定，初步分析了土壤理化因子对可培养细菌群落结构的影响。结果表明在菲尔德斯半岛人活动对土壤中可培养细菌群落的影响显著区别于企鹅和海豹。研究还对产淀粉酶的菌株进行了筛选，发现8株产淀粉酶的菌株。

关键词: 南极, 菲尔德斯半岛, 可培养细菌, 系统进化分析, 土壤理化性质

Abstract: Culturable bacteria were isolated from 16 soil samples collected from Fildes Peninsula during the 29th Chinese Antarctic Scientific Expedition. Classification and phylogenetic analysis based on 16S rDNA showed that the 67 bacteria belonged to 3 classes and 13 genera. The predominant phyla were Actinobacteria and Proteobacteria and the predominant genera were Arthrobacter and Pseudomonas. The soil properties at four typical sites influenced by humans, seals, and penguins were analyzed. Preliminary analyses of the relationships between soil properties and bacterial diversity and community were conducted. The results showed that bacterial diversity and community were affected by humans, seals, and penguins at different levels. Overall, eight amylase-producing bacteria were found in this study.

Key words: Antarctica, Fildes Peninsula, culturable bacteria, phylogenetic analysis, soil properties