极地研究

极地研究

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Phylogenetic analysis and in vitro culture of mosses from the Antarctic Fildes Peninsula

LIU Shenghao1,2, ZHANG Zhaohui1, WANG Nengfei1*, CONG Bailin1, ZHANG Pengying2, LIN Xuezheng1 & HUANG Xiaohang1   

  1. 1 Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, Qingdao 266061, China;
    2 College of Life Science, Shandong University, 27 Shanda Nanlu, Jinan 250100, China
  • 出版日期:1964-06-30 发布日期:1964-06-30
  • 通讯作者: WANG Nengfei

Phylogenetic analysis and in vitro culture of mosses from the Antarctic Fildes Peninsula

LIU Shenghao1,2, ZHANG Zhaohui1, WANG Nengfei1*, CONG Bailin1, ZHANG Pengying2, LIN Xuezheng1 & HUANG Xiaohang1   

  1. 1 Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, Qingdao 266061, China;
    2 College of Life Science, Shandong University, 27 Shanda Nanlu, Jinan 250100, China
  • Online:1964-06-30 Published:1964-06-30
  • Contact: WANG Nengfei

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摘要： Molecular genetic techniques have proven very useful for initial analysis of the extent of genetic variation and dispersal in several Antarctic moss species. In the present study, the small subunit ribosomal RNA (SSU rDNA) and internal transcribed spacers of the nuclear ribosomal DNA (ITS rDNA) were sequenced in nine individuals of different mosses from the Fildes Peninsula of Antarctica. Sequence alignment showed that the extreme environment tended to increase the genetic diversity of Antarctic mosses. In addition, in our phylogenetic analysis, one previously unidentified Antarctic moss species was characterized by comparison with SSU and ITS rDNA sequences of known moss species. Moreover, the optimal culture medium and conditions for surface explant sterilization and protonemata induction in tissue culture of Pohlia nutans were investigated. The successful establishment of a tissue culture protocol together with the phylogenetic analysis of Antarctic mosses will provide technological support to establish an effective resource regeneration method for discovering new functional genes and gaining novel insights into the mechanisms of stress acclimation.

关键词: phylogenetic tree, tissue culture, protonemata formation, Pohlia nutans

Abstract: Molecular genetic techniques have proven very useful for initial analysis of the extent of genetic variation and dispersal in several Antarctic moss species. In the present study, the small subunit ribosomal RNA (SSU rDNA) and internal transcribed spacers of the nuclear ribosomal DNA (ITS rDNA) were sequenced in nine individuals of different mosses from the Fildes Peninsula of Antarctica. Sequence alignment showed that the extreme environment tended to increase the genetic diversity of Antarctic mosses. In addition, in our phylogenetic analysis, one previously unidentified Antarctic moss species was characterized by comparison with SSU and ITS rDNA sequences of known moss species. Moreover, the optimal culture medium and conditions for surface explant sterilization and protonemata induction in tissue culture of Pohlia nutans were investigated. The successful establishment of a tissue culture protocol together with the phylogenetic analysis of Antarctic mosses will provide technological support to establish an effective resource regeneration method for discovering new functional genes and gaining novel insights into the mechanisms of stress acclimation.

Key words: phylogenetic tree, tissue culture, protonemata formation, Pohlia nutans