极地研究

极地研究 ›› 2015, Vol. 27 ›› Issue (3): 246-254.

• 研究论文 • 上一篇    下一篇

2011年夏季北极王湾细菌群落结构分析及浮游细菌丰度检测

乔宗赟1 曾胤新1,2 董培艳3,4 郑天凌3,4   

  1. 1 集美大学食品与工程学院，福建 厦门 361021； 2 国家海洋局极地科学重点实验室，中国极地研究中心，上海 200136； 3 厦门大学生命科学学院，滨海湿地生态系统教育部重点实验室，福建 厦门 361005； 4 厦门大学近海海洋环境科学国家重点实验室，福建 厦门 361005
  • 收稿日期:2014-03-31 修回日期:2014-05-04 出版日期:2015-09-30 发布日期:2015-09-30
  • 通讯作者: 曾胤新
  • 基金资助:

    国家自然科学基金;南北极环境综合考察与评估专项

BACTERIAL COMMUNITY COMPOSITION AND ABUNDANCE OF PLANKTONIC BACTERIA IN ARCTIC KONGSFJORDEN IN THE SUMMER OF 2011

Qiao Zongyun1,2, Zeng Yinxin1,2, Dong Peiyan3,4, Zheng Tianling3,4   

  1. 1 College of Food and Biological Engineering, Jimei University, Xiamen 361021, China； 2 SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China； 3 Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, China 4 State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
  • Received:2014-03-31 Revised:2014-05-04 Online:2015-09-30 Published:2015-09-30
  • Contact: Zeng Yinxin

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被引次数

1

摘要： 本研究采用PCR-DGGE方法，对2011年夏季北极王湾表层海水及沉积物细菌的群落分析结果表明，沉积物中的细菌多样性指数高于海水。深水站位(S1和S3)的沉积物细菌群落不但与浮游细菌群落存在差异，并且与浅水站位(S5)也存在差异。位于湾口、湾内的浮游细菌群落组成也存在一定差异。测序结果显示，王湾海洋细菌的多样性组成包括α-变形细菌、γ-变形细菌、δ-变形细菌、放线菌、拟杆菌及厚壁菌等类群。基于定量PCR方法的检测结果表明，位于湾口、湾内的浮游细菌丰度相似，但湾内玫瑰杆菌支系的丰度明显低于湾口。研究结果表明，与湾口相比，王湾湾内的细菌群落受陆源性淡水输入的影响明显，不但表现在细菌群落的多样性组成上，也表现在某些特定细菌类群的数量分布上。

关键词: 北极王湾, 细菌多样性, PCR-DGGE-丰度定量, PCR

Abstract: Bacteria are one of major components of the marine food web and play an important role in marine ecosystems. High abundance of bacteria has been reported in the Arctic Kongsfjorden. Being a typical Arctic fjord, the Kongsfjorden is part of the north-western coast of Svalbard. Freshwater input from glaciers inevitably affects the bacterial communities in Kongsfjorden during summer season. Community fingerprint analysis of surface water and sediment samples collected in the summer of 2011 was performed by using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach. Sediment bacterial communities were found to show higher diversity index than surface seawater. Due to the influence of terrestrial freshwater input, sediments from station with water deeper than 200 m showed different bacterial communities from surface seawaters, as well as the sediment from station with water shallower than 40 m. Differences in bacterioplankton community compositions were also observed between the inner and outer part of the fjord. Sequences of DGGE bands fell into six major lineages of the domain Bacteria, includingα-,γ- andδ-Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes. In contrast to similar abundance of planktonic bacteria between the outer and inner part of Kongsfjorden on the basis of quantitative PCR (QPCR) assay, higher abundance of Roseobacter clade was detected in the outer part than inner part of the fjord. Results in this study suggest that, compared to outer part, inner part of Kongsfjorden were more affected by terrestrial freshwater input, resulting in differences in bacterial community composition as well as abundance of specific bacterial species. In addition, compared to investigation in the summer of 2007, lower diversity of planktonic bacteria was found in this study. Whether those differences reflect the response of bacterial communities in Kongsfjorden to global climate change remains uncertain.

Key words: Arctic Kongsfjorden, bacterial diversity, PCR-DGGE, PCR

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