利用两种活体荧光技术分析北极微藻对光照变化的响应

doi:10.3724/SP.J.2013.00257

极地研究 ›› 2013, Vol. 25 ›› Issue (3): 257-267.DOI: 10.3724/SP.J.2013.00257

利用两种活体荧光技术分析北极微藻对光照变化的响应

王肖颖^1,2何剑锋¹ 光应芝¹ 李娟英² 张芳¹

¹国家海洋局极地科学重点实验室，中国极地研究中心，上海 200136； ²上海海洋大学水产与生命学院，上海 201306

收稿日期:2013-01-04 修回日期:2013-04-25 出版日期:2013-09-30 发布日期:2013-09-30
通讯作者: 张芳
基金资助:
南、北极海洋微微型浮游植物群落及其生态作用对比研究;北极黄河站冰川融水对近岸海域微型浮游植物群落的调控作用研究

STUDIES ON RESPONSE OF ARCTIC MICROALGAE TO CHANGE OF IRRADIANCE, APPLYING TWO KINDS OF FLUORESCENT TECHNIQUES

Wang Xiaoying^1,2，He Jianfeng¹，Guang Yingzhi¹，Li Juanying²，Zhang Fang¹

¹ SOA Key Laboratory for Polar Science,Polar Research Institute of China, Shanghai 200136, China; ² College of Fisheries and life science, Shanghai Ocean University, Shanghai 201306,China

Received:2013-01-04 Revised:2013-04-25 Online:2013-09-30 Published:2013-09-30
Contact: Fang Zhang

摘要/Abstract

摘要： 采用调制叶绿素荧光技术（脉冲-振幅-调制：Pulse-Amplitude-Modulation，简称为PAM技术）和荧光光谱两种技术研究不同光照下的两种北极微藻（北极异帽藻（Heterocapsa arctica）和红胞藻（Rhodomonas sp.）的荧光特性，发现（1）两种藻在三个不同光照水平下（5 000 lux、15 000 lux、25 000 lux）光合生理变化特征相似：随着光照强度增加，光量子产量越低，光合效率越低；在15 000 lux和25 000 lux两个较强光照条件下，最大光合电子传递速率（rETRmax）没有提高，且捕光能力下降；25 000 lux光照强度下，均处于胁迫状态，但对强光的耐受能力增强；（2）两种藻在15 000 lux条件下Chl a荧光强度最高，25 000 lux条件下的Chl a荧光强度都显著降低，受到不同程度的光损伤；类胡萝卜素和多甲藻素是维持北极异帽藻15 000 lux下种群生存的有效色素；藻胆素和Chl c则分别为维持红胞藻5 000 lux和15 000 lux条件下的种群的生存起了积极作用。

关键词: 北极微藻, 光照, 调制叶绿素荧光, 荧光光谱

Abstract: This work investigated fluorescence characteristic of two kinds of arctic algae (Heterocapsa arctica and Rhodomonas sp.) by two kinds of fluorescence techniques in vivo including Pulse-Amplitude-Modulation (PAM) and fluorescence spectrum. The main results are as follows: (1) the photosynthetic physiology of two kinds of algae were similar under the three kinds of irradiance (5000 lux 15000 lux and 25000 lux); the irradiance is stronger, the value of the Fv/Fm and photosynthetic efficiency are higher; under 25000 lux, they were in stress state, but their endurance capacity was enhanced. (2) the fluorescence intensity of Chl a of the two kinds of algae is highest under 15000lux; the fluorescence intensity of Chl a reduced on 15000lux; Carotenoids and Peridinins were the effective pigments for the survival of Heterocapsa arctica under 15000lux; phycobilin and Chl c had a positive effect on maintain the existence of Rhodomonas sp. under 5000 lux and 15000 lux.

Key words: Arctic microalgae, irradiance, pulse-amplitude-modulation(PAM), ChlorophyⅡ, fluorometer, fluorescence spectrum

王肖颖何剑锋光应芝李娟英张芳. 利用两种活体荧光技术分析北极微藻对光照变化的响应[J]. 极地研究, 2013, 25(3): 257-267.

Wang Xiaoying，He Jianfeng，Guang Yingzhi，Li Juanying，Zhang Fang. STUDIES ON RESPONSE OF ARCTIC MICROALGAE TO CHANGE OF IRRADIANCE, APPLYING TWO KINDS OF FLUORESCENT TECHNIQUES[J]. ADVANCES IN POLAR SCIENCE, 2013, 25(3): 257-267.

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利用两种活体荧光技术分析北极微藻对光照变化的响应

STUDIES ON RESPONSE OF ARCTIC MICROALGAE TO CHANGE OF IRRADIANCE, APPLYING TWO KINDS OF FLUORESCENT TECHNIQUES

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