极地研究

极地研究 ›› 2011, Vol. 23 ›› Issue (2): 108-114.DOI: 10.3724/SP.J.1084.2011.00108

• 研究论文 • 上一篇    下一篇

一株分离自北极海冰的产β-半乳糖苷酶的海单胞菌（Marinomonas sp. BSi20414）的筛选、鉴定和产酶条件研究

曾倩1,2 王毓舒1,2 孙鲲2 俞勇2 陈波2   

  1. 1华东理工大学生物工程学院；
    2 中国极地研究中心国家海洋局极地科学重点实验室
  • 收稿日期:2011-01-10 修回日期:2011-02-18 出版日期:2011-06-30 发布日期:2011-06-30
  • 通讯作者: 陈波
  • 基金资助:

    海洋公益性行业科研专项经费项目;中国极地科学战略研究基金

Preliminary studies on the screening，identification and optimum fermentative conditions of a strain Marinomonas sp.BSi20414 isolated from arctic sea ice producing β-galactosidase

  • Received:2011-01-10 Revised:2011-02-18 Online:2011-06-30 Published:2011-06-30

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摘要：

从北极海冰中分离筛选出一株产β-半乳糖苷酶的菌株BSi20414，经初步鉴定，该菌株归属于海单胞属(Marinomonas)，命名为Marinomonas sp. BSi20414。对该菌株的产酶条件实验表明，最适产酶条件为：30℃，180 rpm摇瓶培养96 h ，培养基中加1.5%（w/v）乳糖，初始pH 7.0 ，接种量3% ，500 mL三角瓶装产酶培养基80 mL。在该条件下酶活由复筛阶段的1.01 U/mL提高到条件优化后的2.92 U/mL。实验初步表明粗酶液对ONPG底物的最适作用温度为60℃，推测该菌株具有低温菌产高温酶的可能特点。

关键词: 产酶条件

Abstract:

A strain BSi20414 with higher β-galactosidase activity was isolated from Arctic sea ice. Based on its Physiological and biochemical characteristics and 16S rDNA sequence, it was identified as Marinomonas sp. BSi20414. A series of the detail experiments on the conditions of β-galactosidase production were conducted and the results showed that the optimal fermentation conditions for enzyme production were: 30℃，1.5% (w/v) lactose, initial pH 7.0，Inoculum amount 3%，80 mL fermentative medium in 500 mL flask, 180 rpm for 96 hrs. Under these conditions, the enzyme activity of 1.01 U / mL in the second screening stage increased to 2.92 U / mL. Preliminary experiments showed that the optimal reaction temperature of the crude enzyme on ONPG substrate was 60 ℃. This Result suggested that this cold adapted strain Marinomonas sp. BSi20414 may be have a characteristic of producing high-temperature enzyme.

Key words: fermentation conditions